MSF Field Research >
1 Published Research and Commentary >
Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa.
this identifier to cite or link
to this item:
|Download this article:|
|Chaillet P et al RDT HIV 1 and 2 Transactions.pdf||416Kb||Adobe PDF|
|Title: ||Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa.|
|Affiliation: ||Médecins sans Frontiérès, Medical Department, Brussels Operational Center, Brussels, Belgium.|
|Citation: ||Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa. 2010, 104 (9):571-6 Trans. R. Soc. Trop. Med. Hyg.|
|Journal: ||Transactions of the Royal Society of Tropical Medicine and Hygiene|
|Issue Date: ||Sep-2010 |
|PubMed ID: ||20708123|
|Abstract: ||With both HIV-1 and HV-2 prevalent in Guinea-Conakry, accurate diagnosis and differentiation is crucial for treatment purposes. Thus, four rapid HIV tests were evaluated for their HIV-1 and HIV-2 diagnostic and discriminative capacity for use in Guinea-Conakry. These included SD Bioline HIV 1/2 3.0 (Standard Diagnostics Inc.), Genie II HIV1/HIV2 (Bio-Rad), First Response HIV Card Test 1-2.0 (PMC Medical) and Immunoflow HIV1-HIV2 (Core Diagnostics). Results were compared with gold standard tests (INNO-LIA HIV-I/II Score) and NEW LAV BLOT II (Bio-Rad). Four hundred and forty three sequential stored HIV-positive serum samples, of known HIV-type, were evaluated. Genie II HIV1/HIV2, Immunoflow HIV1-HIV2 and SD Bioline HIV 1/2 3.0 had 100% sensitivity (95% CI, 98.9-100%) while for First Response HIV Card Test 1-2.0 this was 99.5% (95% CI, 98.2%-99.9%). In terms of discriminatory capacity, Genie II HIV1/HIV2 identified 382/ 384(99.5%) HIV-1 samples, 49/ 52(95%) HIV-2 and 7/7(100%) HIV-positive untypable samples. Immunoflow HIV1-HIV2 identified 99% HIV-1, 67% HIV-2 and all HIV-positive untypable samples. First Response HIV Card Test 1-2.0 identified 94% HIV-1, 64% HIV-2 and 57% HIV-positive untypable samples. SD-Bioline HIV 1/2 3.0 was the worst overall performer identifying 65% HIV-1, 69% HIV-2 and all HIV-positive untypable samples. The use of SD Bioline HIV 1/2 3.0 (the current standard in Guinea-Conakry) as a discriminatory HIV test is poor and may be best replaced by Immunoflow HIV1-HIV2.|
|Rights: ||Published by Elsevier
Archived on this site with the kind permission of Elsevier Ltd. (http://www.sciencedirect.com/science/journal/00359203) and the Royal Society of Tropical Medicine and Hygiene (http://www.rstmh.org/transactions.asp)|
|Appears in topics: ||Laboratory|
|Related articles on PubMed|
Performance of 3 rapid tests for discrimination between HIV-1 and HIV-2 in Guinea-Bissau, West Africa.
Hønge BL, Bjarnason Obinah MP, Jespersen S, Medina C, Té Dda S, da Silva ZJ, Østergaard L, Laursen AL, Wejse C, Erikstrup C, Bissau HIV Cohort Study Group
2014 Jan 1
|See all 101 articles|
All Items in MSF are protected by copyright, with all rights reserved, unless otherwise indicated.