MSF Field Research > 1 Published Research and Commentary > Laboratory > Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa.

Please use this identifier to cite or link to this item: http://hdl.handle.net/10144/112714
    Del.icio.us     LinkedIn     Citeulike     Connotea     Facebook     Stumble it!


Download this article:
View/Open File Description Size Format
Thumbnail
View/Open
Chaillet P et al RDT HIV 1 and 2 Transactions.pdf416KbAdobe PDF

Title: Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa.
Authors: Chaillet, Pascale
Tayler-Smith, Katie
Zachariah, Rony
Duclos, Nanfack
Moctar, Diallo
Beelaert, Greet
Fransen, Katrien
Affiliation: Médecins sans Frontiérès, Medical Department, Brussels Operational Center, Brussels, Belgium.
Citation: Evaluation of four rapid tests for diagnosis and differentiation of HIV-1 and HIV-2 infections in Guinea-Conakry, West Africa. 2010, 104 (9):571-6 Trans. R. Soc. Trop. Med. Hyg.
Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene
Issue Date: Sep-2010
URI: http://hdl.handle.net/10144/112714
DOI: 10.1016/j.trstmh.2010.05.007
PubMed ID: 20708123
Abstract: With both HIV-1 and HV-2 prevalent in Guinea-Conakry, accurate diagnosis and differentiation is crucial for treatment purposes. Thus, four rapid HIV tests were evaluated for their HIV-1 and HIV-2 diagnostic and discriminative capacity for use in Guinea-Conakry. These included SD Bioline HIV 1/2 3.0 (Standard Diagnostics Inc.), Genie II HIV1/HIV2 (Bio-Rad), First Response HIV Card Test 1-2.0 (PMC Medical) and Immunoflow HIV1-HIV2 (Core Diagnostics). Results were compared with gold standard tests (INNO-LIA HIV-I/II Score) and NEW LAV BLOT II (Bio-Rad). Four hundred and forty three sequential stored HIV-positive serum samples, of known HIV-type, were evaluated. Genie II HIV1/HIV2, Immunoflow HIV1-HIV2 and SD Bioline HIV 1/2 3.0 had 100% sensitivity (95% CI, 98.9-100%) while for First Response HIV Card Test 1-2.0 this was 99.5% (95% CI, 98.2%-99.9%). In terms of discriminatory capacity, Genie II HIV1/HIV2 identified 382/ 384(99.5%) HIV-1 samples, 49/ 52(95%) HIV-2 and 7/7(100%) HIV-positive untypable samples. Immunoflow HIV1-HIV2 identified 99% HIV-1, 67% HIV-2 and all HIV-positive untypable samples. First Response HIV Card Test 1-2.0 identified 94% HIV-1, 64% HIV-2 and 57% HIV-positive untypable samples. SD-Bioline HIV 1/2 3.0 was the worst overall performer identifying 65% HIV-1, 69% HIV-2 and all HIV-positive untypable samples. The use of SD Bioline HIV 1/2 3.0 (the current standard in Guinea-Conakry) as a discriminatory HIV test is poor and may be best replaced by Immunoflow HIV1-HIV2.
Language: en
ISSN: 1878-3503
Rights: Published by Elsevier Archived on this site with the kind permission of Elsevier Ltd. (http://www.sciencedirect.com/science/journal/00359203) and the Royal Society of Tropical Medicine and Hygiene (http://www.rstmh.org/transactions.asp)
Appears in topics: Laboratory

Related articles on PubMed
bullet
Evaluation of simple rapid HIV assays and development of national rapid HIV test algorithms in Dar es Salaam, Tanzania.
Lyamuya EF, Aboud S, Urassa WK, Sufi J, Mbwana J, Ndugulile F, Massambu C
2009 Feb 18
bullet
Performance of 3 rapid tests for discrimination between HIV-1 and HIV-2 in Guinea-Bissau, West Africa.
Hønge BL, Bjarnason Obinah MP, Jespersen S, Medina C, Té Dda S, da Silva ZJ, Østergaard L, Laursen AL, Wejse C, Erikstrup C, Bissau HIV Cohort Study Group
2014 Jan 1
bullet
bullet
bullet
See all 99 articles

All Items in MSF are protected by copyright, with all rights reserved, unless otherwise indicated.

 

OR Logo Powered by Open Repository | Cookies

My MSF

MSF logo MSF Field Research

Sep 2, 2014