Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates

Hdl Handle:
http://hdl.handle.net/10144/116329
Title:
Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates
Authors:
Martin, A; Paasch, F; Von Groll, A; Fissette, K; Almeida, P; Varaine, F; Portaels, F; Palomino, J-C
Journal:
The International Journal of Tuberculosis and Lung Disease : the official journal of the International Union against Tuberculosis and Lung Disease
Abstract:
BACKGROUND: In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics. OBJECTIVE: To evaluate the thin-layer agar (TLA) for rapid detection of resistance to rifampicin (RMP), ofloxacin (OFX) and kanamycin (KM) in M. tuberculosis clinical isolates and to determine the sensitivity, specificity and time to positivity compared to the gold standard method. METHODS: One hundred and forty-seven clinical isolates of M. tuberculosis were studied. For the TLA method, a quadrant Petri plate containing 7H11 agar with RMP, OFX and KM was used. Results were compared to the Bactec MGIT960 for RMP and the proportion method for OFX and KM. RESULTS: The sensitivity and specificity for RMP and OFX were 100% and for KM they were 100% and 98.7%, respectively. The use of a TLA quadrant plate enables the rapid detection of resistance to the three anti-tuberculosis drugs RMP, OFX and KM in a median of 10 days. CONCLUSION: TLA was an accurate method for the detection of resistance in the three drugs studied. This faster method is simple to perform, providing an alternative method when more sophisticated techniques are not available in low-resource settings.
Affiliation:
Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium; Laboratory of Mycobacteriology, Universidade Federal do Rio Grande, Rio Grande do Sul, Brazil; Médecins Sans Frontières, Paris, France
Issue Date:
1-Oct-2009
URI:
http://hdl.handle.net/10144/116329
PubMed ID:
19793437
Additional Links:
http://www.ingentaconnect.com/content/iuatld/ijtld/2009/00000013/00000010/art00019?token=004b10b9383a4b3b2570232b2f5f7338432c2079662a726e2d2954496f642f466f0498d5d9d
Type:
Article
Language:
en
ISSN:
1815-7920
Appears in Collections:
TB

Full metadata record

DC FieldValue Language
dc.contributor.authorMartin, Aen
dc.contributor.authorPaasch, Fen
dc.contributor.authorVon Groll, Aen
dc.contributor.authorFissette, Ken
dc.contributor.authorAlmeida, Pen
dc.contributor.authorVaraine, Fen
dc.contributor.authorPortaels, Fen
dc.contributor.authorPalomino, J-Cen
dc.date.accessioned2010-11-25T15:53:44Z-
dc.date.available2010-11-25T15:53:44Z-
dc.date.issued2009-10-01-
dc.identifier.citationInt. J. Tuberc. Lung Dis. 2009;13(10):1301-4en
dc.identifier.issn1815-7920-
dc.identifier.pmid19793437-
dc.identifier.urihttp://hdl.handle.net/10144/116329-
dc.description.abstractBACKGROUND: In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics. OBJECTIVE: To evaluate the thin-layer agar (TLA) for rapid detection of resistance to rifampicin (RMP), ofloxacin (OFX) and kanamycin (KM) in M. tuberculosis clinical isolates and to determine the sensitivity, specificity and time to positivity compared to the gold standard method. METHODS: One hundred and forty-seven clinical isolates of M. tuberculosis were studied. For the TLA method, a quadrant Petri plate containing 7H11 agar with RMP, OFX and KM was used. Results were compared to the Bactec MGIT960 for RMP and the proportion method for OFX and KM. RESULTS: The sensitivity and specificity for RMP and OFX were 100% and for KM they were 100% and 98.7%, respectively. The use of a TLA quadrant plate enables the rapid detection of resistance to the three anti-tuberculosis drugs RMP, OFX and KM in a median of 10 days. CONCLUSION: TLA was an accurate method for the detection of resistance in the three drugs studied. This faster method is simple to perform, providing an alternative method when more sophisticated techniques are not available in low-resource settings.en
dc.language.isoenen
dc.relation.urlhttp://www.ingentaconnect.com/content/iuatld/ijtld/2009/00000013/00000010/art00019?token=004b10b9383a4b3b2570232b2f5f7338432c2079662a726e2d2954496f642f466f0498d5d9den
dc.rightsArchived with thanks to The International Journal of Tuberculosis and Lung Disease : the official journal of the International Union against Tuberculosis and Lung Diseaseen
dc.subject.meshAgaren
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshAntitubercular Agentsen
dc.subject.meshBacteriological Techniquesen
dc.subject.meshDrug Resistance, Bacterialen
dc.subject.meshHumansen
dc.subject.meshKanamycinen
dc.subject.meshMicrobial Sensitivity Testsen
dc.subject.meshMycobacterium tuberculosisen
dc.subject.meshOfloxacinen
dc.subject.meshRifampinen
dc.subject.meshSensitivity and Specificityen
dc.titleThin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolatesen
dc.typeArticleen
dc.contributor.departmentInstitute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium; Laboratory of Mycobacteriology, Universidade Federal do Rio Grande, Rio Grande do Sul, Brazil; Médecins Sans Frontières, Paris, Franceen
dc.identifier.journalThe International Journal of Tuberculosis and Lung Disease : the official journal of the International Union against Tuberculosis and Lung Diseaseen

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