Transmission of Plasmodium vivax in South-Western Uganda: Report of Three Cases in Pregnant Women

Hdl Handle:
http://hdl.handle.net/10144/141434
Title:
Transmission of Plasmodium vivax in South-Western Uganda: Report of Three Cases in Pregnant Women
Authors:
Dhorda, Mehul; Nyehangane, Dan; Rénia, Laurent; Piola, Patrice; Guerin, Philippe J; Snounou, Georges
Journal:
PloS One
Abstract:
Plasmodium vivax is considered to be rare in the predominantly Duffy negative populations of Sub-Saharan Africa, as this red blood cell surface antigen is essential for invasion by the parasite. However, despite only very few reports of molecularly confirmed P. vivax from tropical Africa, serological evidence indicated that 13% of the persons sampled in Congo had been exposed to P. vivax. We identified P. vivax by microscopy in 8 smears from Ugandan pregnant women who had been enrolled in a longitudinal study of malaria in pregnancy. A nested polymerase chain reaction (PCR) protocol was used to detect and identify the Plasmodium parasites present. PCR analysis confirmed the presence of P. vivax for three of the women and analysis of all available samples from these women revealed clinically silent chronic low-grade vivax infections for two of them. The parasites in one woman carried pyrimethamine resistance-associated double non-synonymous mutations in the P. vivax dihydrofolate reductase gene. The three women found infected with P. vivax were Duffy positive as were nine of 68 women randomly selected from the cohort. The data presented from these three case reports is consistent with stable transmission of malaria in a predominantly Duffy negative African population. Given the substantial morbidity associated with vivax infection in non-African endemic areas, it will be important to investigate whether the distribution and prevalence of P. vivax have been underestimated in Sub-Saharan Africa. This is particularly important in the context of the drive to eliminate malaria and its morbidity.
Affiliation:
Epicentre, Mbarara, Uganda; Institut National de la Sante´ et de la Recherche Medicale,Paris, France; Universite´ Pierre et Marie Curie, Faculte´ de Me´decine Pitie´-Salpeˆ trie` re, Paris, France; Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore; Epicentre, Paris, France; Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, United Kingdom
Issue Date:
13-May-2011
URI:
http://hdl.handle.net/10144/141434
DOI:
10.1371/journal.pone.0019801
PubMed ID:
21603649
Additional Links:
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019801
Submitted date:
2011-05-26
Type:
Article
Language:
en
ISSN:
1932-6203
Appears in Collections:
Malaria

Full metadata record

DC FieldValue Language
dc.contributor.authorDhorda, Mehulen
dc.contributor.authorNyehangane, Danen
dc.contributor.authorRénia, Laurenten
dc.contributor.authorPiola, Patriceen
dc.contributor.authorGuerin, Philippe Jen
dc.contributor.authorSnounou, Georgesen
dc.date.accessioned2011-08-31T19:03:44Z-
dc.date.available2011-08-31T19:03:44Z-
dc.date.issued2011-05-13-
dc.date.submitted2011-05-26-
dc.identifier.citationPLoS ONE 2011;6(5):e19801en
dc.identifier.issn1932-6203-
dc.identifier.pmid21603649-
dc.identifier.doi10.1371/journal.pone.0019801-
dc.identifier.urihttp://hdl.handle.net/10144/141434-
dc.description.abstractPlasmodium vivax is considered to be rare in the predominantly Duffy negative populations of Sub-Saharan Africa, as this red blood cell surface antigen is essential for invasion by the parasite. However, despite only very few reports of molecularly confirmed P. vivax from tropical Africa, serological evidence indicated that 13% of the persons sampled in Congo had been exposed to P. vivax. We identified P. vivax by microscopy in 8 smears from Ugandan pregnant women who had been enrolled in a longitudinal study of malaria in pregnancy. A nested polymerase chain reaction (PCR) protocol was used to detect and identify the Plasmodium parasites present. PCR analysis confirmed the presence of P. vivax for three of the women and analysis of all available samples from these women revealed clinically silent chronic low-grade vivax infections for two of them. The parasites in one woman carried pyrimethamine resistance-associated double non-synonymous mutations in the P. vivax dihydrofolate reductase gene. The three women found infected with P. vivax were Duffy positive as were nine of 68 women randomly selected from the cohort. The data presented from these three case reports is consistent with stable transmission of malaria in a predominantly Duffy negative African population. Given the substantial morbidity associated with vivax infection in non-African endemic areas, it will be important to investigate whether the distribution and prevalence of P. vivax have been underestimated in Sub-Saharan Africa. This is particularly important in the context of the drive to eliminate malaria and its morbidity.en
dc.language.isoenen
dc.relation.urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0019801en
dc.rightsPublished by Public Library of Science, [url]http://www.plosone.org/[/url] Archived on this site by Open Access permissionen
dc.subject.meshMalaria, Vivaxen
dc.subject.meshDisease Transmission, communicableen
dc.subject.meshPregnant Womenen
dc.subject.meshDuffy blood group protein, humanen
dc.titleTransmission of Plasmodium vivax in South-Western Uganda: Report of Three Cases in Pregnant Womenen
dc.typeArticleen
dc.contributor.departmentEpicentre, Mbarara, Uganda; Institut National de la Sante´ et de la Recherche Medicale,Paris, France; Universite´ Pierre et Marie Curie, Faculte´ de Me´decine Pitie´-Salpeˆ trie` re, Paris, France; Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR), Biopolis, Singapore; Epicentre, Paris, France; Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, United Kingdomen
dc.identifier.journalPloS Oneen

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