Evaluation of a New Recombinant K39 Rapid Diagnostic Test for Sudanese Visceral Leishmaniasis.

Hdl Handle:
http://hdl.handle.net/10144/17289
Title:
Evaluation of a New Recombinant K39 Rapid Diagnostic Test for Sudanese Visceral Leishmaniasis.
Authors:
Ritmeijer, K; Melaku, Y; Mueller, M; Kipngetich, S; O'keeffe, C; Davidson, R N
Journal:
The American Journal of Tropical Medicine and Hygiene
Abstract:
A new rK39 rapid diagnostic dipstick test (DiaMed-IT-Leish) was compared with aspiration and a direct agglutination test (DAT) for diagnosis of visceral leishmaniasis (VL) in 201 parasitologically confirmed cases, 133 endemic controls, and in 356 clinical suspects in disease-endemic and -epidemic areas in Sudan. The sensitivity of the rK39 test in parasitologically confirmed VL cases was 90%, whereas the specificity in disease-endemic controls was 99%. The sensitivity of the DAT was 98%. In clinically suspected cases, the sensitivity of the rK39 test was 81% and the specificity was 97%. When compared with the diagnostic protocol based on the DAT and aspiration used by Médecins sans Frontières in epidemic situations, the positive predictive value was 98%, and the negative predictive value was 71%. This rK39 rapid diagnostic test is suitable for screening as well as diagnosis of VL. Further diagnostic work-up of dipstick-negative patients with clinically suspected VL is important. The ease and convenience of the dipstick test will allow decentralization and improved access to care in disease-endemic areas in Sudan.
Affiliation:
Médecins sans Frontières-Holland, Amsterdam, The Netherlands. koert.ritmeijer@amsterdam.msf.org
Publisher:
Published by: American Society of Tropical Medicine and Hygiene
Issue Date:
Jan-2006
URI:
http://hdl.handle.net/10144/17289
PubMed ID:
16407349
Additional Links:
http://www.ajtmh.org
Language:
en
ISSN:
0002-9637
Appears in Collections:
Leishmaniasis/Kala Azar

Full metadata record

DC FieldValue Language
dc.contributor.authorRitmeijer, K-
dc.contributor.authorMelaku, Y-
dc.contributor.authorMueller, M-
dc.contributor.authorKipngetich, S-
dc.contributor.authorO'keeffe, C-
dc.contributor.authorDavidson, R N-
dc.date.accessioned2008-01-31T16:40:39Z-
dc.date.available2008-01-31T16:40:39Z-
dc.date.issued2006-01-
dc.identifier.citationEvaluation of a New Recombinant K39 Rapid Diagnostic Test for Sudanese Visceral Leishmaniasis. 2006, 74 (1):76-80 Am. J. Trop. Med. Hyg.en
dc.identifier.issn0002-9637-
dc.identifier.pmid16407349-
dc.identifier.urihttp://hdl.handle.net/10144/17289-
dc.description.abstractA new rK39 rapid diagnostic dipstick test (DiaMed-IT-Leish) was compared with aspiration and a direct agglutination test (DAT) for diagnosis of visceral leishmaniasis (VL) in 201 parasitologically confirmed cases, 133 endemic controls, and in 356 clinical suspects in disease-endemic and -epidemic areas in Sudan. The sensitivity of the rK39 test in parasitologically confirmed VL cases was 90%, whereas the specificity in disease-endemic controls was 99%. The sensitivity of the DAT was 98%. In clinically suspected cases, the sensitivity of the rK39 test was 81% and the specificity was 97%. When compared with the diagnostic protocol based on the DAT and aspiration used by Médecins sans Frontières in epidemic situations, the positive predictive value was 98%, and the negative predictive value was 71%. This rK39 rapid diagnostic test is suitable for screening as well as diagnosis of VL. Further diagnostic work-up of dipstick-negative patients with clinically suspected VL is important. The ease and convenience of the dipstick test will allow decentralization and improved access to care in disease-endemic areas in Sudan.en
dc.language.isoenen
dc.publisherPublished by: American Society of Tropical Medicine and Hygiene-
dc.relation.urlhttp://www.ajtmh.org-
dc.rightsArchived on this site with the kind permission of the American Society of Tropical Medicine and Hygiene, www.astmh.orgen
dc.subject.meshAntigens, Protozoanen
dc.subject.meshHumansen
dc.subject.meshLeishmaniasis, Visceralen
dc.subject.meshPredictive Value of Testsen
dc.subject.meshProtozoan Proteinsen
dc.subject.meshReagent Kits, Diagnosticen
dc.subject.meshRecombinant Proteinsen
dc.subject.meshSensitivity and Specificityen
dc.subject.meshSudanen
dc.subject.meshTime Factorsen
dc.titleEvaluation of a New Recombinant K39 Rapid Diagnostic Test for Sudanese Visceral Leishmaniasis.en
dc.contributor.departmentMédecins sans Frontières-Holland, Amsterdam, The Netherlands. koert.ritmeijer@amsterdam.msf.orgen
dc.identifier.journalThe American Journal of Tropical Medicine and Hygieneen
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