Paracheck-Pf accuracy and recently treated Plasmodium falciparum infections: is there a risk of over-diagnosis?

Hdl Handle:
http://hdl.handle.net/10144/17738
Title:
Paracheck-Pf accuracy and recently treated Plasmodium falciparum infections: is there a risk of over-diagnosis?
Authors:
Swarthout, T D; Counihan, H; Senga, R K K; van den Broek, I
Journal:
Malaria Journal
Abstract:
BACKGROUND: An assessment of the accuracy of Paracheck Pf, a malaria rapid diagnostic test (RDT) detecting histidine rich protein 2 was undertaken amongst children aged 6-59 months in eastern Democratic Republic of Congo. METHODS: This RDT assessment occurred in conjunction with an ACT efficacy trial. Febrile children were simultaneously screened with both RDT and high quality microscopy and those meeting inclusion criteria were followed for 35 days. RESULTS: 358 febrile children were screened with 180 children recruited for five weeks follow-up. On screening, the RDT accurately diagnosed all 235 true malaria cases, indicating 100% RDT sensitivity. Of the 123 negative slides, the RDT gave 59 false-positive results, indicating 52.0% (64/123) RDT specificity. During follow-up after treatment with an artemisinin-based combination therapy, 98.2% (110/112), 94.6% (106/112), 92.0% (103/112) and 73.5% (50/68) of effectively treated children were still false-positive by RDT at days 14, 21, 28 and 35, respectively. CONCLUSION: Results show that though the use of Paracheck-Pf is as sensitive as microscopy in detecting true malaria cases, a low specificity did present a high frequency of false-positive RDT results. What's more, a duration of RDT false-positivity was found that significantly surpassed the 'fortnight' after effective treatment reported by its manufacturer. Though further research is needed in assessing RDT accuracy, study results showing the presence of frequent false positivity should be taken into consideration to avoid clinicians inappropriately focusing on malaria, not identifying the true cause of illness, and providing unnecessary treatment.
Affiliation:
Médecins Sans Frontières, London, UK. toddswarth@yahoo.com
Publisher:
Elsevier
Issue Date:
2007
URI:
http://hdl.handle.net/10144/17738
DOI:
10.1186/1475-2875-6-58
PubMed ID:
17506881
Additional Links:
http://www.thelancet.com
Language:
en
ISSN:
1475-2875
Appears in Collections:
Malaria

Full metadata record

DC FieldValue Language
dc.contributor.authorSwarthout, T D-
dc.contributor.authorCounihan, H-
dc.contributor.authorSenga, R K K-
dc.contributor.authorvan den Broek, I-
dc.date.accessioned2008-02-07T16:41:17Z-
dc.date.available2008-02-07T16:41:17Z-
dc.date.issued2007-
dc.identifier.citationParacheck-Pf accuracy and recently treated Plasmodium falciparum infections: is there a risk of over-diagnosis? 2007, 6:58 Malar. J.en
dc.identifier.issn1475-2875-
dc.identifier.pmid17506881-
dc.identifier.doi10.1186/1475-2875-6-58-
dc.identifier.urihttp://hdl.handle.net/10144/17738-
dc.description.abstractBACKGROUND: An assessment of the accuracy of Paracheck Pf, a malaria rapid diagnostic test (RDT) detecting histidine rich protein 2 was undertaken amongst children aged 6-59 months in eastern Democratic Republic of Congo. METHODS: This RDT assessment occurred in conjunction with an ACT efficacy trial. Febrile children were simultaneously screened with both RDT and high quality microscopy and those meeting inclusion criteria were followed for 35 days. RESULTS: 358 febrile children were screened with 180 children recruited for five weeks follow-up. On screening, the RDT accurately diagnosed all 235 true malaria cases, indicating 100% RDT sensitivity. Of the 123 negative slides, the RDT gave 59 false-positive results, indicating 52.0% (64/123) RDT specificity. During follow-up after treatment with an artemisinin-based combination therapy, 98.2% (110/112), 94.6% (106/112), 92.0% (103/112) and 73.5% (50/68) of effectively treated children were still false-positive by RDT at days 14, 21, 28 and 35, respectively. CONCLUSION: Results show that though the use of Paracheck-Pf is as sensitive as microscopy in detecting true malaria cases, a low specificity did present a high frequency of false-positive RDT results. What's more, a duration of RDT false-positivity was found that significantly surpassed the 'fortnight' after effective treatment reported by its manufacturer. Though further research is needed in assessing RDT accuracy, study results showing the presence of frequent false positivity should be taken into consideration to avoid clinicians inappropriately focusing on malaria, not identifying the true cause of illness, and providing unnecessary treatment.en
dc.language.isoenen
dc.publisherElsevier-
dc.relation.urlhttp://www.thelancet.com-
dc.rightsArchived on this site by Open Access permissionen
dc.subject.meshAnimalsen
dc.subject.meshChild, Preschoolen
dc.subject.meshCongoen
dc.subject.meshFalse Positive Reactionsen
dc.subject.meshHumansen
dc.subject.meshInfanten
dc.subject.meshMalaria, Falciparumen
dc.subject.meshPlasmodium falciparumen
dc.subject.meshReagent Kits, Diagnosticen
dc.subject.meshSensitivity and Specificityen
dc.titleParacheck-Pf accuracy and recently treated Plasmodium falciparum infections: is there a risk of over-diagnosis?en
dc.contributor.departmentMédecins Sans Frontières, London, UK. toddswarth@yahoo.comen
dc.identifier.journalMalaria Journalen

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