The Partec CyFlow Counter could provide an option for CD4+ T-cell monitoring in the context of scaling-up antiretroviral treatment at the district level in Malawi.

Hdl Handle:
http://hdl.handle.net/10144/17740
Title:
The Partec CyFlow Counter could provide an option for CD4+ T-cell monitoring in the context of scaling-up antiretroviral treatment at the district level in Malawi.
Authors:
Fryland, M; Chaillet, P; Zachariah, R; Barnaba, A; Bonte, L; Andereassen, R; Charrondière, S; Teck, R; Didakus, O
Journal:
Transactions of the Royal Society of Tropical Medicine and Hygiene
Abstract:
A study was conducted in rural Malawi to verify (a) whether the Partec CyFlow Counter((R)) for CD4+ T-cell lymphocyte counting in HIV-positive individuals could be introduced into a district hospital laboratory and (b) whether it would produce CD4 counts of acceptable quality. CD4+ cell counting was performed using the Partec CyFlow Counter and the results were compared with a reference method (FACsCount). A total of 311 blood samples were analysed and the correlation coefficient for the CyFlow Counter was 0.92 (95% CI 0.89-0.95). Mean CD4 counts using the Partec and the reference methods were 308.2 cells/microl and 316.9 cells/microl, respectively. The mean difference in CD4 count values was -8.68 cells/microl (95% CI -18.8 to 1.4). Mean intra-run variation was -6.84 cells/microl (95% CI -12.9 to 0.79). In the district laboratory setting, the instrument could accommodate up to 75 blood samples per technician per day. After being trained, local laboratory staff found the CyFlow Counter procedures simple to run and the instrument easy to manipulate. The Partec CyFlow Counter produces sufficiently reliable results and the instrument appears robust under field conditions. It could provide a new option for introducing routine CD4+ cell monitoring at the district level in the context of scaling-up antiretroviral therapy in Malawi.
Affiliation:
Médecins sans Frontières-Luxembourg, Thyolo District, Malawi.
Publisher:
Elsevier
Issue Date:
Oct-2006
URI:
http://hdl.handle.net/10144/17740
DOI:
10.1016/j.trstmh.2005.11.014
PubMed ID:
16542690
Additional Links:
http://www.sciencedirect.com/science/journal/00359203
Language:
en
ISSN:
0035-9203
Appears in Collections:
HIV/AIDS

Full metadata record

DC FieldValue Language
dc.contributor.authorFryland, M-
dc.contributor.authorChaillet, P-
dc.contributor.authorZachariah, R-
dc.contributor.authorBarnaba, A-
dc.contributor.authorBonte, L-
dc.contributor.authorAndereassen, R-
dc.contributor.authorCharrondière, S-
dc.contributor.authorTeck, R-
dc.contributor.authorDidakus, O-
dc.date.accessioned2008-02-07T16:43:29Z-
dc.date.available2008-02-07T16:43:29Z-
dc.date.issued2006-10-
dc.identifier.citationThe Partec CyFlow Counter could provide an option for CD4+ T-cell monitoring in the context of scaling-up antiretroviral treatment at the district level in Malawi. 2006, 100 (10):980-5 Trans. R. Soc. Trop. Med. Hyg.en
dc.identifier.issn0035-9203-
dc.identifier.pmid16542690-
dc.identifier.doi10.1016/j.trstmh.2005.11.014-
dc.identifier.urihttp://hdl.handle.net/10144/17740-
dc.description.abstractA study was conducted in rural Malawi to verify (a) whether the Partec CyFlow Counter((R)) for CD4+ T-cell lymphocyte counting in HIV-positive individuals could be introduced into a district hospital laboratory and (b) whether it would produce CD4 counts of acceptable quality. CD4+ cell counting was performed using the Partec CyFlow Counter and the results were compared with a reference method (FACsCount). A total of 311 blood samples were analysed and the correlation coefficient for the CyFlow Counter was 0.92 (95% CI 0.89-0.95). Mean CD4 counts using the Partec and the reference methods were 308.2 cells/microl and 316.9 cells/microl, respectively. The mean difference in CD4 count values was -8.68 cells/microl (95% CI -18.8 to 1.4). Mean intra-run variation was -6.84 cells/microl (95% CI -12.9 to 0.79). In the district laboratory setting, the instrument could accommodate up to 75 blood samples per technician per day. After being trained, local laboratory staff found the CyFlow Counter procedures simple to run and the instrument easy to manipulate. The Partec CyFlow Counter produces sufficiently reliable results and the instrument appears robust under field conditions. It could provide a new option for introducing routine CD4+ cell monitoring at the district level in the context of scaling-up antiretroviral therapy in Malawi.en
dc.language.isoenen
dc.publisherElsevier-
dc.relation.urlhttp://www.sciencedirect.com/science/journal/00359203-
dc.rightsArchived on this site with the kind permission of Elsevier Ltd. and the Royal Society of Tropical Medicine and Hygiene, http://www.rstmh.org/transactions.aspen
dc.subject.meshAntiretroviral Therapy, Highly Activeen
dc.subject.meshCD4 Lymphocyte Counten
dc.subject.meshCD4-Positive T-Lymphocytesen
dc.subject.meshFlow Cytometryen
dc.subject.meshHIV Infectionsen
dc.subject.meshHospitals, Districten
dc.subject.meshHumansen
dc.subject.meshRural Healthen
dc.subject.meshSensitivity and Specificityen
dc.titleThe Partec CyFlow Counter could provide an option for CD4+ T-cell monitoring in the context of scaling-up antiretroviral treatment at the district level in Malawi.en
dc.contributor.departmentMédecins sans Frontières-Luxembourg, Thyolo District, Malawi.en
dc.identifier.journalTransactions of the Royal Society of Tropical Medicine and Hygieneen

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