Short Report: Molecular Markers Associated with Plasmodium Falciparum Resistance to Sulfadoxine-Pyrimethamine in the Democratic Republic of Congo.

Hdl Handle:
http://hdl.handle.net/10144/18354
Title:
Short Report: Molecular Markers Associated with Plasmodium Falciparum Resistance to Sulfadoxine-Pyrimethamine in the Democratic Republic of Congo.
Authors:
Cohuet, S; Bonnet, M; Van Herp, M; Van Overmeir, C; D'Alessandro, U; Guthmann, J P P
Journal:
The American Journal of Tropical Medicine and Hygiene
Abstract:
Sulfadoxine-pyrimethamine (SP) is the first line antimalarial treatment in the Democratic Republic of Congo. Using polymerase chain reaction, we assessed the prevalence of mutations in the dihydrofolate reductase (dhfr) (codons 108, 51, 59) and dihydropteroate synthase (dhps) (codons 437, 540) genes of Plasmodium falciparum, which have been associated with resistance to pyrimethamine and sulfadoxine, respectively. Four hundred seventy-four patients were sampled in Kilwa (N = 138), Kisangani (N = 112), Boende (N = 106), and Basankusu (N = 118). The proportion of triple mutations dhfr varied between sites but was always > 50%. The proportion of dhps double mutations was < 20%, with some sites as low as 0.9%. A quintuple mutation was present in 12.8% (16/125) samples in Kilwa; 11.9% (13/109) in Kisangani, 2.9% (3/102) in Boende, and 0.9% (1/112) in Basankusu. These results suggest high resistance to pyrimethamine alone or combined with sulfadoxine. Adding artesunate to SP does not seem a valid alternative to the current monotherapy.
Affiliation:
Epicentre, Paris, France; Médecins Sans Frontières, Brussels, Belgium; Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium.
Issue Date:
Jul-2006
URI:
http://hdl.handle.net/10144/18354
PubMed ID:
16837723
Additional Links:
http://www.ajtmh.org; http://www.ajtmh.org
Language:
en
ISSN:
0002-9637
Appears in Collections:
Laboratory

Full metadata record

DC FieldValue Language
dc.contributor.authorCohuet, S-
dc.contributor.authorBonnet, M-
dc.contributor.authorVan Herp, M-
dc.contributor.authorVan Overmeir, C-
dc.contributor.authorD'Alessandro, U-
dc.contributor.authorGuthmann, J P P-
dc.date.accessioned2008-02-14T15:57:01Z-
dc.date.available2008-02-14T15:57:01Z-
dc.date.issued2006-07-
dc.identifier.citationShort Report: Molecular Markers Associated with Plasmodium Falciparum Resistance to Sulfadoxine-Pyrimethamine in the Democratic Republic of Congo. 2006, 75 (1):152-4 Am. J. Trop. Med. Hyg.en
dc.identifier.issn0002-9637-
dc.identifier.pmid16837723-
dc.identifier.urihttp://hdl.handle.net/10144/18354-
dc.description.abstractSulfadoxine-pyrimethamine (SP) is the first line antimalarial treatment in the Democratic Republic of Congo. Using polymerase chain reaction, we assessed the prevalence of mutations in the dihydrofolate reductase (dhfr) (codons 108, 51, 59) and dihydropteroate synthase (dhps) (codons 437, 540) genes of Plasmodium falciparum, which have been associated with resistance to pyrimethamine and sulfadoxine, respectively. Four hundred seventy-four patients were sampled in Kilwa (N = 138), Kisangani (N = 112), Boende (N = 106), and Basankusu (N = 118). The proportion of triple mutations dhfr varied between sites but was always > 50%. The proportion of dhps double mutations was < 20%, with some sites as low as 0.9%. A quintuple mutation was present in 12.8% (16/125) samples in Kilwa; 11.9% (13/109) in Kisangani, 2.9% (3/102) in Boende, and 0.9% (1/112) in Basankusu. These results suggest high resistance to pyrimethamine alone or combined with sulfadoxine. Adding artesunate to SP does not seem a valid alternative to the current monotherapy.en
dc.language.isoenen
dc.relation.urlhttp://www.ajtmh.org-
dc.relation.urlhttp://www.ajtmh.org-
dc.rightsArchived on this site with the kind permission of the American Society of Tropical Medicine and Hygiene, www.astmh.orgen
dc.subject.meshAnimalsen
dc.subject.meshAntimalarialsen
dc.subject.meshCodonen
dc.subject.meshDemocratic Republic of the Congoen
dc.subject.meshDihydropteroate Synthaseen
dc.subject.meshDrug Combinationsen
dc.subject.meshDrug Resistanceen
dc.subject.meshGenetic Markersen
dc.subject.meshGenotypeen
dc.subject.meshHumansen
dc.subject.meshMutationen
dc.subject.meshPlasmodium falciparumen
dc.subject.meshPolymerase Chain Reactionen
dc.subject.meshPrevalenceen
dc.subject.meshPyrimethamineen
dc.subject.meshSulfadoxineen
dc.subject.meshTetrahydrofolate Dehydrogenaseen
dc.titleShort Report: Molecular Markers Associated with Plasmodium Falciparum Resistance to Sulfadoxine-Pyrimethamine in the Democratic Republic of Congo.en
dc.contributor.departmentEpicentre, Paris, France; Médecins Sans Frontières, Brussels, Belgium; Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium.en
dc.identifier.journalThe American Journal of Tropical Medicine and Hygieneen

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