Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria.

Hdl Handle:
http://hdl.handle.net/10144/18393
Title:
Assessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria.
Authors:
Fogg, C; Twesigye, R; Batwala, V; Piola, P; Nabasumba, C; Kiguli, J; Mutebi, F; Hook, C; Guillerm, M; Moody, A; Guthmann, J P
Journal:
Transactions of the Royal Society of Tropical Medicine and Hygiene
Abstract:
A study to assess the diagnostic capabilities of three parasite lactate dehydrogenase (pan-pLDH) tests, Vistapan), Carestart and Parabank), was conducted in Uganda. An HRP2 test, Paracheck-Pf), and a Giemsa-stained blood film were performed with the pLDH tests for outpatients with suspected malaria. In total, 460 subjects were recruited: 248 with positive blood films and 212 with negative blood films. Plasmodium falciparum was present in 95% of infections. Sensitivity above 90% was shown by two pLDH tests, Carestart (95.6%) and Vistapan (91.9%), and specificity above 90% by Parabank (94.3%) and Carestart (91.5%). Sensitivity decreased with low parasitaemia (chi(2) trend, P<0.001); however, all tests achieved sensitivity >90% with parasitaemia > or =100/microl. All tests had good inter-reader reliability (kappa>0.95). Two weeks after diagnosis, 4-10% of pLDH tests were still positive compared with 69.7% of the HRP2 tests. All tests had similar ease of use. In conclusion, two pLDH tests performed well in diagnosing P. falciparum malaria, and all pLDH tests became negative after treatment more quickly than the HRP2. Therefore the rapid test of choice for use with artemisinin-combination therapies in this area would be one of these new pLDH tests.
Affiliation:
Epicentre, 8 rue Saint Sabin, 75011, Paris, France. carolefogg@yahoo.co.uk
Publisher:
Elsevier
Issue Date:
Jan-2008
URI:
http://hdl.handle.net/10144/18393
DOI:
10.1016/j.trstmh.2007.09.014
PubMed ID:
18031779
Additional Links:
http://www.sciencedirect.com/science/journal/00359203
Language:
en
ISSN:
0035-9203
Appears in Collections:
Malaria

Full metadata record

DC FieldValue Language
dc.contributor.authorFogg, C-
dc.contributor.authorTwesigye, R-
dc.contributor.authorBatwala, V-
dc.contributor.authorPiola, P-
dc.contributor.authorNabasumba, C-
dc.contributor.authorKiguli, J-
dc.contributor.authorMutebi, F-
dc.contributor.authorHook, C-
dc.contributor.authorGuillerm, M-
dc.contributor.authorMoody, A-
dc.contributor.authorGuthmann, J P-
dc.date.accessioned2008-02-14T16:30:02Z-
dc.date.available2008-02-14T16:30:02Z-
dc.date.issued2008-01-
dc.identifier.citationAssessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria. 2008, 102 (1):25-31 Trans. R. Soc. Trop. Med. Hyg.en
dc.identifier.issn0035-9203-
dc.identifier.pmid18031779-
dc.identifier.doi10.1016/j.trstmh.2007.09.014-
dc.identifier.urihttp://hdl.handle.net/10144/18393-
dc.description.abstractA study to assess the diagnostic capabilities of three parasite lactate dehydrogenase (pan-pLDH) tests, Vistapan), Carestart and Parabank), was conducted in Uganda. An HRP2 test, Paracheck-Pf), and a Giemsa-stained blood film were performed with the pLDH tests for outpatients with suspected malaria. In total, 460 subjects were recruited: 248 with positive blood films and 212 with negative blood films. Plasmodium falciparum was present in 95% of infections. Sensitivity above 90% was shown by two pLDH tests, Carestart (95.6%) and Vistapan (91.9%), and specificity above 90% by Parabank (94.3%) and Carestart (91.5%). Sensitivity decreased with low parasitaemia (chi(2) trend, P<0.001); however, all tests achieved sensitivity >90% with parasitaemia > or =100/microl. All tests had good inter-reader reliability (kappa>0.95). Two weeks after diagnosis, 4-10% of pLDH tests were still positive compared with 69.7% of the HRP2 tests. All tests had similar ease of use. In conclusion, two pLDH tests performed well in diagnosing P. falciparum malaria, and all pLDH tests became negative after treatment more quickly than the HRP2. Therefore the rapid test of choice for use with artemisinin-combination therapies in this area would be one of these new pLDH tests.en
dc.language.isoenen
dc.publisherElsevier-
dc.relation.urlhttp://www.sciencedirect.com/science/journal/00359203-
dc.rightsArchived on this site with the kind permission of Elsevier Ltd. and the Royal Society of Tropical Medicine and Hygiene, http://www.rstmh.org/transactions.aspen
dc.subject.meshMalariaen
dc.subject.meshDiagnosisen
dc.subject.meshrapid diagnostic testsen
dc.subject.meshvalidityen
dc.subject.meshSensitivity and specificityen
dc.subject.meshParasite lactate dehydrogenase testsen
dc.titleAssessment of three new parasite lactate dehydrogenase (pan-pLDH) tests for diagnosis of uncomplicated malaria.en
dc.contributor.departmentEpicentre, 8 rue Saint Sabin, 75011, Paris, France. carolefogg@yahoo.co.uken
dc.identifier.journalTransactions of the Royal Society of Tropical Medicine and Hygieneen

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