Characterization of the Chromosomal Aminoglycoside 2'-N-Acetyltransferase Gene from Mycobacterium Fortuitum.

Hdl Handle:
http://hdl.handle.net/10144/18395
Title:
Characterization of the Chromosomal Aminoglycoside 2'-N-Acetyltransferase Gene from Mycobacterium Fortuitum.
Authors:
Aínsa, J; Martin, C; Gicquel, B; Gomez-Lus, R
Journal:
Antimicrobial Agents and Chemotherapy
Abstract:
A novel gene encoding an aminoglycoside 2'-N-acetyltransferase (AAC) was cloned from Mycobacterium fortuitum. DNA sequencing results identified an open reading frame that we have called aac(2')-Ib encoding a putative protein with a predicted molecular mass of 24,800 Da. The deduced AAC(2')-Ib protein showed homology to the AAC(2')-Ia from Providencia stuartii. This is the second member of a subfamily of AAC(2')-I enzymes to be identified. No homology was found with other acetyltransferases, including all of the AAC(3) and AAC(6') proteins. The aac(2')-Ib gene cloned in a mycobacterial plasmid and introduced in Mycobacterium smegmatis conferred resistance to gentamicin, tobramycin, dibekacin, netilmicin, and 6'-N-ethylnetilmicin. DNA hybridization with an intragenic probe of aac(2')-Ib showed that this gene was present in all 34 strains of M. fortuitum tested. The universal presence of the aac(2')-Ib gene in M. fortuitum was not correlated with any aminoglycoside resistance phenotype, suggesting that this gene may play a role in the secondary metabolism of the bacterium.
Affiliation:
Departamento de Microbiología, Facultad de Medicina, Universidad de Zaragoza, Spain. Jose.Ainsa@msf.unizar.es
Publisher:
Published by American Society for Microbiology
Issue Date:
Oct-1996
URI:
http://hdl.handle.net/10144/18395
PubMed ID:
8891143
Language:
en
ISSN:
0066-4804
Appears in Collections:
Laboratory

Full metadata record

DC FieldValue Language
dc.contributor.authorAínsa, J-
dc.contributor.authorMartin, C-
dc.contributor.authorGicquel, B-
dc.contributor.authorGomez-Lus, R-
dc.date.accessioned2008-02-14T16:42:10Z-
dc.date.available2008-02-14T16:42:10Z-
dc.date.issued1996-10-
dc.identifier.citationCharacterization of the Chromosomal Aminoglycoside 2'-N-Acetyltransferase Gene from Mycobacterium Fortuitum. 1996, 40 (10):2350-5 Antimicrob. Agents Chemother.en
dc.identifier.issn0066-4804-
dc.identifier.pmid8891143-
dc.identifier.urihttp://hdl.handle.net/10144/18395-
dc.description.abstractA novel gene encoding an aminoglycoside 2'-N-acetyltransferase (AAC) was cloned from Mycobacterium fortuitum. DNA sequencing results identified an open reading frame that we have called aac(2')-Ib encoding a putative protein with a predicted molecular mass of 24,800 Da. The deduced AAC(2')-Ib protein showed homology to the AAC(2')-Ia from Providencia stuartii. This is the second member of a subfamily of AAC(2')-I enzymes to be identified. No homology was found with other acetyltransferases, including all of the AAC(3) and AAC(6') proteins. The aac(2')-Ib gene cloned in a mycobacterial plasmid and introduced in Mycobacterium smegmatis conferred resistance to gentamicin, tobramycin, dibekacin, netilmicin, and 6'-N-ethylnetilmicin. DNA hybridization with an intragenic probe of aac(2')-Ib showed that this gene was present in all 34 strains of M. fortuitum tested. The universal presence of the aac(2')-Ib gene in M. fortuitum was not correlated with any aminoglycoside resistance phenotype, suggesting that this gene may play a role in the secondary metabolism of the bacterium.en
dc.language.isoenen
dc.publisherPublished by American Society for Microbiology-
dc.rightsArchived on this site with permission and copyright by the American Society for Microbiologyen
dc.subject.meshAcetyltransferasesen
dc.subject.meshAmino Acid Sequenceen
dc.subject.meshAminoglycosidesen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshBase Sequenceen
dc.subject.meshChromosomes, Bacterialen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDrug Resistance, Microbialen
dc.subject.meshEscherichia colien
dc.subject.meshMicrobial Sensitivity Testsen
dc.subject.meshMolecular Sequence Dataen
dc.subject.meshMutationen
dc.subject.meshMycobacteriumen
dc.subject.meshNucleic Acid Hybridizationen
dc.subject.meshPlasmidsen
dc.subject.meshProvidenciaen
dc.titleCharacterization of the Chromosomal Aminoglycoside 2'-N-Acetyltransferase Gene from Mycobacterium Fortuitum.en
dc.contributor.departmentDepartamento de Microbiología, Facultad de Medicina, Universidad de Zaragoza, Spain. Jose.Ainsa@msf.unizar.esen
dc.identifier.journalAntimicrobial Agents and Chemotherapyen
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