Suppression of circulating IgD+CD27+ memory B cells in infants living in a malaria-endemic region of Kenya

Hdl Handle:
http://hdl.handle.net/10144/213269
Title:
Suppression of circulating IgD+CD27+ memory B cells in infants living in a malaria-endemic region of Kenya
Authors:
Asito, Amolo S; Piriou, Erwan; Jura, Walter GZO; Ouma, Collins; Odada, Peter S; Ogola, Sidney; Fiore, Nancy; Rochford, Rosemary
Journal:
Malaria Journal
Abstract:
Background: Plasmodium falciparum infection leads to alterations in B cell subset distribution. During infancy, development of peripheral B cell subsets is also occurring. However, it is unknown if infants living a malaria endemic region have alterations in B cell subsets that is independent of an age effect. Methods: To evaluate the impact of exposure to P. falciparum on B cell development in infants, flow cytometry was used to analyse the distribution and phenotypic characteristic of B cell subsets in infant cohorts prospectively followed at 12, 18 and 24 months from two geographically proximate regions in western Kenya with divergent malaria exposure i.e. Kisumu (malaria-endemic, n = 24) and Nandi (unstable malaria transmission, n = 21). Results: There was significantly higher frequency and absolute cell numbers of CD19+ B cells in Kisumu relative to Nandi at 12(p = 0.0440), 18(p = 0.0210) and 24 months (p = 0.0493). No differences were observed between the infants from the two sites in frequencies of naïve B cells (IgD+CD27-) or classical memory B cells (IgD-CD27+). However, immature transitional B cells (CD19+CD10+CD34-) were higher in Kisumu relative to Nandi at all three ages. In contrast, the levels of non-class switched memory B cells (CD19+IgD+CD27+) were significantly lower overall in Kisumu relative to Nandi at significantly at 12 (p = 0.0144), 18 (p = 0.0013) and 24 months (p = 0.0129). Conclusions: These data suggest that infants living in malaria endemic regions have altered B cell subset distribution. Further studies are needed to understand the functional significance of these changes and long-term impact on ability of these infants to develop antibody responses to P. falciparum and heterologous infections.
Affiliation:
Maseno University, Maseno, Kenya; Center for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya; SUNY Upstate Medical University, Syracuse, NY; Medecins Sans Frontieres-Operational Centre Amsterdam, Amsterdam, The Netherlands
Publisher:
BioMed Central
Issue Date:
13-Dec-2011
URI:
http://hdl.handle.net/10144/213269
DOI:
10.1186/1475-2875-10-362
Additional Links:
http://www.malariajournal.com/content/10/1/362
Type:
Article
Language:
en
ISSN:
1475-2875
Appears in Collections:
Malaria

Full metadata record

DC FieldValue Language
dc.contributor.authorAsito, Amolo Sen
dc.contributor.authorPiriou, Erwanen
dc.contributor.authorJura, Walter GZOen
dc.contributor.authorOuma, Collinsen
dc.contributor.authorOdada, Peter Sen
dc.contributor.authorOgola, Sidneyen
dc.contributor.authorFiore, Nancyen
dc.contributor.authorRochford, Rosemaryen
dc.date.accessioned2012-02-28T06:33:59Z-
dc.date.available2012-02-28T06:33:59Z-
dc.date.issued2011-12-13-
dc.identifier.citationMal J 2011; 10:362en
dc.identifier.issn1475-2875-
dc.identifier.doi10.1186/1475-2875-10-362-
dc.identifier.urihttp://hdl.handle.net/10144/213269-
dc.description.abstractBackground: Plasmodium falciparum infection leads to alterations in B cell subset distribution. During infancy, development of peripheral B cell subsets is also occurring. However, it is unknown if infants living a malaria endemic region have alterations in B cell subsets that is independent of an age effect. Methods: To evaluate the impact of exposure to P. falciparum on B cell development in infants, flow cytometry was used to analyse the distribution and phenotypic characteristic of B cell subsets in infant cohorts prospectively followed at 12, 18 and 24 months from two geographically proximate regions in western Kenya with divergent malaria exposure i.e. Kisumu (malaria-endemic, n = 24) and Nandi (unstable malaria transmission, n = 21). Results: There was significantly higher frequency and absolute cell numbers of CD19+ B cells in Kisumu relative to Nandi at 12(p = 0.0440), 18(p = 0.0210) and 24 months (p = 0.0493). No differences were observed between the infants from the two sites in frequencies of naïve B cells (IgD+CD27-) or classical memory B cells (IgD-CD27+). However, immature transitional B cells (CD19+CD10+CD34-) were higher in Kisumu relative to Nandi at all three ages. In contrast, the levels of non-class switched memory B cells (CD19+IgD+CD27+) were significantly lower overall in Kisumu relative to Nandi at significantly at 12 (p = 0.0144), 18 (p = 0.0013) and 24 months (p = 0.0129). Conclusions: These data suggest that infants living in malaria endemic regions have altered B cell subset distribution. Further studies are needed to understand the functional significance of these changes and long-term impact on ability of these infants to develop antibody responses to P. falciparum and heterologous infections.en
dc.language.isoenen
dc.publisherBioMed Centralen
dc.relation.urlhttp://www.malariajournal.com/content/10/1/362en
dc.rightsPublished by BioMed Central, [url]http://www.malariajournal.com/[/url] Archived on this site by Open Access permissionen
dc.subject.meshMalariaen
dc.subject.meshInfanten
dc.titleSuppression of circulating IgD+CD27+ memory B cells in infants living in a malaria-endemic region of Kenyaen
dc.typeArticleen
dc.contributor.departmentMaseno University, Maseno, Kenya; Center for Global Health Research, Kenya Medical Research Institute, Kisumu, Kenya; SUNY Upstate Medical University, Syracuse, NY; Medecins Sans Frontieres-Operational Centre Amsterdam, Amsterdam, The Netherlandsen
dc.identifier.journalMalaria Journalen
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