Performance of a Histidine-Rich Protein 2 Rapid Diagnostic Test, Paracheck Pf(R), for Detection of Malaria Infections in Ugandan Pregnant Women.

Hdl Handle:
http://hdl.handle.net/10144/220351
Title:
Performance of a Histidine-Rich Protein 2 Rapid Diagnostic Test, Paracheck Pf(R), for Detection of Malaria Infections in Ugandan Pregnant Women.
Authors:
Dhorda, Mehul; Piola, Patrice; Nyehangane, Dan; Tumwebaze, Benon; Nalusaji, Aisha; Nabasumba, Carolyn; Turyakira, Eleanor; McGready, Rose; Ashley, Elizabeth; Guerin, Philippe J; Snounou, Georges
Journal:
The American Journal of Tropical Medicine and Hygiene
Abstract:
Abstract. Improved laboratory diagnosis is critical to reduce the burden of malaria in pregnancy. Peripheral blood smears appear less sensitive than Plasmodium falciparum histidine-rich protein 2-based rapid diagnostic tests (RDTs) for placental malaria infections in studies conducted at delivery. In this study, 81 women in Uganda in the second or third trimester of pregnancy were followed-up until delivery. At each visit, peripheral blood was tested by blood smear, RDT, and nested species-specific polymerase chain reaction (PCR). Sensitivity and specificity of the tests was calculated with PCR, which detected 22 infections of P. falciparum, as the gold standard. The sensitivity and specificity of blood smears were 36.4% (95% confidence interval [CI] = 18.0-59.2%) and 99.6% (95% CI = 97.7-100%), respectively. The corresponding values for RDT were 31.8% (95% CI = 14.7-54.9%) and 100% (95% CI = 98.3-100%). The RDTs could replace blood smears for diagnosis of malaria in pregnancy by virtue of their relative ease of use. Field-based sensitive tests for malaria in pregnancy are urgently needed.
Affiliation:
Epicentre, Mbarara, Uganda; Unité Mixte de Recherche 945, Institut National de la Santé et de la Recherche Médicale, Paris, France; Université Pierre et Marie Curie, Faculté de Médecine Pitié-Salpêtrière, Paris, France; Epicentre, Paris, France; Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom; Mbarara University of Science and Technology, Mbarara, Uganda; Shoklo Malaria Research Unit, Mae Sot, Tak, Thailand.
Publisher:
Am.J.trop.Med.Hyg.
Issue Date:
1-Jan-2012
URI:
http://hdl.handle.net/10144/220351
DOI:
10.4269/ajtmh.2012.10-0631
PubMed ID:
22232456
Additional Links:
http://www.ajtmh.org
Type:
Article
Language:
en
ISSN:
1476-1645
Appears in Collections:
Malaria

Full metadata record

DC FieldValue Language
dc.contributor.authorDhorda, Mehulen_GB
dc.contributor.authorPiola, Patriceen_GB
dc.contributor.authorNyehangane, Danen_GB
dc.contributor.authorTumwebaze, Benonen_GB
dc.contributor.authorNalusaji, Aishaen_GB
dc.contributor.authorNabasumba, Carolynen_GB
dc.contributor.authorTuryakira, Eleanoren_GB
dc.contributor.authorMcGready, Roseen_GB
dc.contributor.authorAshley, Elizabethen_GB
dc.contributor.authorGuerin, Philippe Jen_GB
dc.contributor.authorSnounou, Georgesen_GB
dc.date.accessioned2012-04-24T16:28:26Z-
dc.date.available2012-04-24T16:28:26Z-
dc.date.issued2012-01-01-
dc.identifier.citationPerformance of a Histidine-Rich Protein 2 Rapid Diagnostic Test, Paracheck Pf(R), for Detection of Malaria Infections in Ugandan Pregnant Women. 2012, 86 (1):93-95notAm J Trop Med Hygen_GB
dc.identifier.issn1476-1645-
dc.identifier.pmid22232456-
dc.identifier.doi10.4269/ajtmh.2012.10-0631-
dc.identifier.urihttp://hdl.handle.net/10144/220351-
dc.description.abstractAbstract. Improved laboratory diagnosis is critical to reduce the burden of malaria in pregnancy. Peripheral blood smears appear less sensitive than Plasmodium falciparum histidine-rich protein 2-based rapid diagnostic tests (RDTs) for placental malaria infections in studies conducted at delivery. In this study, 81 women in Uganda in the second or third trimester of pregnancy were followed-up until delivery. At each visit, peripheral blood was tested by blood smear, RDT, and nested species-specific polymerase chain reaction (PCR). Sensitivity and specificity of the tests was calculated with PCR, which detected 22 infections of P. falciparum, as the gold standard. The sensitivity and specificity of blood smears were 36.4% (95% confidence interval [CI] = 18.0-59.2%) and 99.6% (95% CI = 97.7-100%), respectively. The corresponding values for RDT were 31.8% (95% CI = 14.7-54.9%) and 100% (95% CI = 98.3-100%). The RDTs could replace blood smears for diagnosis of malaria in pregnancy by virtue of their relative ease of use. Field-based sensitive tests for malaria in pregnancy are urgently needed.en_GB
dc.languageENG-
dc.language.isoenen
dc.publisherAm.J.trop.Med.Hyg.en_GB
dc.relation.urlhttp://www.ajtmh.orgen_GB
dc.rightsArchived with thanks to The American Journal of Tropical Medicine and Hygieneen_GB
dc.subjectMalariaen_GB
dc.subjectdiagnostic testen_GB
dc.subjectParachecken_GB
dc.subjectUgandaen_GB
dc.titlePerformance of a Histidine-Rich Protein 2 Rapid Diagnostic Test, Paracheck Pf(R), for Detection of Malaria Infections in Ugandan Pregnant Women.en
dc.typeArticleen
dc.contributor.departmentEpicentre, Mbarara, Uganda; Unité Mixte de Recherche 945, Institut National de la Santé et de la Recherche Médicale, Paris, France; Université Pierre et Marie Curie, Faculté de Médecine Pitié-Salpêtrière, Paris, France; Epicentre, Paris, France; Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, United Kingdom; Mbarara University of Science and Technology, Mbarara, Uganda; Shoklo Malaria Research Unit, Mae Sot, Tak, Thailand.en_GB
dc.identifier.journalThe American Journal of Tropical Medicine and Hygieneen_GB

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