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Title: Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up.
Authors: Schramm, Birgit
Hewison, Cathy
Bonte, Laurence
Jones, Warren
Camélique, Olivier
Ruangweerayut, Ronnatrai
Swaddiwudhipong, Witaya
Bonnet, Maryline
Affiliation: Epicentre, Paris, France; Médecins Sans Frontières (MSF), Paris, France;International Organization for Migration (IOM), Bangkok, Thailand; Epicentre, Geneva, Switzerlandf
Citation: J. Clin. Microbiol 2012; 50(8): 2788-2790.
Journal: Journal of clinical microbiology
Issue Date: Aug-2012
URI: http://hdl.handle.net/10144/255358
DOI: 10.1128/JCM.01232-12
PubMed ID: 22649015
Abstract: Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture.
Type: Article
Language: en
Keywords: Tuberculosis
ISSN: 1098-660X
Rights: Published by the American Society for Microbiology - Archived on this site with kind permission from the American Society for Microbiology
Appears in topics: TB

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