Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up.

Hdl Handle:
http://hdl.handle.net/10144/255358
Title:
Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up.
Authors:
Schramm, Birgit; Hewison, Cathy; Bonte, Laurence; Jones, Warren; Camélique, Olivier; Ruangweerayut, Ronnatrai; Swaddiwudhipong, Witaya; Bonnet, Maryline
Journal:
Journal of clinical microbiology
Abstract:
Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture.
Affiliation:
Epicentre, Paris, France; Médecins Sans Frontières (MSF), Paris, France;International Organization for Migration (IOM), Bangkok, Thailand; Epicentre, Geneva, Switzerlandf
Issue Date:
Aug-2012
URI:
http://hdl.handle.net/10144/255358
DOI:
10.1128/JCM.01232-12
PubMed ID:
22649015
Type:
Article
Language:
en
ISSN:
1098-660X
Appears in Collections:
TB

Full metadata record

DC FieldValue Language
dc.contributor.authorSchramm, Birgiten_GB
dc.contributor.authorHewison, Cathyen_GB
dc.contributor.authorBonte, Laurenceen_GB
dc.contributor.authorJones, Warrenen_GB
dc.contributor.authorCamélique, Olivieren_GB
dc.contributor.authorRuangweerayut, Ronnatraien_GB
dc.contributor.authorSwaddiwudhipong, Witayaen_GB
dc.contributor.authorBonnet, Marylineen_GB
dc.date.accessioned2012-12-11T21:06:44Z-
dc.date.available2012-12-11T21:06:44Z-
dc.date.issued2012-08-
dc.identifier.citationJ. Clin. Microbiol 2012; 50(8): 2788-2790.en_GB
dc.identifier.issn1098-660X-
dc.identifier.pmid22649015-
dc.identifier.doi10.1128/JCM.01232-12-
dc.identifier.urihttp://hdl.handle.net/10144/255358-
dc.description.abstractSimple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture.en_GB
dc.language.isoenen
dc.rightsPublished by the American Society for Microbiology - Archived on this site with kind permission from the American Society for Microbiologyen_GB
dc.subjectTuberculosisen_GB
dc.titleField evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up.en
dc.typeArticleen
dc.contributor.departmentEpicentre, Paris, France; Médecins Sans Frontières (MSF), Paris, France;International Organization for Migration (IOM), Bangkok, Thailand; Epicentre, Geneva, Switzerlandfen_GB
dc.identifier.journalJournal of clinical microbiologyen_GB
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