Molecular epidemiology of multidrug-resistant Shigella dysenteriae type 1 causing dysentery outbreaks in Central African Republic, 2003-2004.

Hdl Handle:
http://hdl.handle.net/10144/31332
Title:
Molecular epidemiology of multidrug-resistant Shigella dysenteriae type 1 causing dysentery outbreaks in Central African Republic, 2003-2004.
Authors:
Bercion, R; Demartin, M; Recio, C; Massamba, P M; Frank, T; Escribà, J M; Grimont, F; Grimont, P A D; Weill, F X
Journal:
Transactions of the Royal Society of Tropical Medicine and Hygiene
Abstract:
Shigella dysenteriae type 1 (Sd1) represents a particular threat in developing countries because of the severity of the infection and its epidemic potential. Antimicrobial susceptibility testing and molecular subtyping by pulsed-field gel electrophoresis (PFGE) and plasmid profiling (PP) of Sd1 isolates collected during two dysentery outbreaks (2013 and 445 cases of bloody diarrhoea) in Central African Republic (CAR) during the period 2003-2004 were reported. Eleven Sd1 comparison strains (CS) acquired by travellers or residents of Africa (n=10) or Asia (n=1) between 1993 and 2003 were also analysed. The 19 Sd1 isolates recovered from CAR outbreaks were multidrug resistant, although susceptible to quinolones and fluoroquinolones. Molecular subtyping by PFGE was more discriminatory than PP. The PFGE using XbaI and NotI restriction enzymes indicated that the two outbreaks were due to two different clones and also revealed a genetic diversity among the CS recovered from outbreak or sporadic cases between 1993 and 2003. This study was the result of a fruitful collaboration between field physicians and microbiologists. The data collected will serve as the basis for establishing long-term monitoring of Sd1 in CAR.
Affiliation:
Institut Pasteur de Bangui, BP 923, Bangui, Central African Republic.
Issue Date:
Dec-2006
URI:
http://hdl.handle.net/10144/31332
DOI:
10.1016/j.trstmh.2006.02.007
PubMed ID:
16701761
Language:
en
ISSN:
0035-9203
Appears in Collections:
Other Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorBercion, R-
dc.contributor.authorDemartin, M-
dc.contributor.authorRecio, C-
dc.contributor.authorMassamba, P M-
dc.contributor.authorFrank, T-
dc.contributor.authorEscribà, J M-
dc.contributor.authorGrimont, F-
dc.contributor.authorGrimont, P A D-
dc.contributor.authorWeill, F X-
dc.date.accessioned2008-07-08T15:17:34Z-
dc.date.available2008-07-08T15:17:34Z-
dc.date.issued2006-12-
dc.identifier.citationMolecular epidemiology of multidrug-resistant Shigella dysenteriae type 1 causing dysentery outbreaks in Central African Republic, 2003-2004. 2006, 100 (12):1151-8 Trans. R. Soc. Trop. Med. Hyg.en
dc.identifier.issn0035-9203-
dc.identifier.pmid16701761-
dc.identifier.doi10.1016/j.trstmh.2006.02.007-
dc.identifier.urihttp://hdl.handle.net/10144/31332-
dc.description.abstractShigella dysenteriae type 1 (Sd1) represents a particular threat in developing countries because of the severity of the infection and its epidemic potential. Antimicrobial susceptibility testing and molecular subtyping by pulsed-field gel electrophoresis (PFGE) and plasmid profiling (PP) of Sd1 isolates collected during two dysentery outbreaks (2013 and 445 cases of bloody diarrhoea) in Central African Republic (CAR) during the period 2003-2004 were reported. Eleven Sd1 comparison strains (CS) acquired by travellers or residents of Africa (n=10) or Asia (n=1) between 1993 and 2003 were also analysed. The 19 Sd1 isolates recovered from CAR outbreaks were multidrug resistant, although susceptible to quinolones and fluoroquinolones. Molecular subtyping by PFGE was more discriminatory than PP. The PFGE using XbaI and NotI restriction enzymes indicated that the two outbreaks were due to two different clones and also revealed a genetic diversity among the CS recovered from outbreak or sporadic cases between 1993 and 2003. This study was the result of a fruitful collaboration between field physicians and microbiologists. The data collected will serve as the basis for establishing long-term monitoring of Sd1 in CAR.en
dc.language.isoenen
dc.rightsPublished by Elsevier Archived on this site with the kind permission of Elsevier Ltd. ([url]http://www.sciencedirect.com/science/journal/00359203[/url]) and the Royal Society of Tropical Medicine and Hygiene ([url]http://www.rstmh.org/transactions.asp[/url])en
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshCentral African Republicen
dc.subject.meshDNA, Bacterialen
dc.subject.meshDisease Outbreaksen
dc.subject.meshDrug Resistance, Multiple, Bacterialen
dc.subject.meshDysentery, Bacillaryen
dc.subject.meshElectrophoresis, Gel, Pulsed-Fielden
dc.subject.meshFemaleen
dc.subject.meshHumansen
dc.subject.meshMaleen
dc.subject.meshPhenotypeen
dc.subject.meshPlasmidsen
dc.titleMolecular epidemiology of multidrug-resistant Shigella dysenteriae type 1 causing dysentery outbreaks in Central African Republic, 2003-2004.en
dc.contributor.departmentInstitut Pasteur de Bangui, BP 923, Bangui, Central African Republic.en
dc.identifier.journalTransactions of the Royal Society of Tropical Medicine and Hygieneen

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