Independent Origin of Plasmodium falciparum Antifolate Super-Resistance, Uganda, Tanzania, and Ethiopia.

Hdl Handle:
http://hdl.handle.net/10144/325834
Title:
Independent Origin of Plasmodium falciparum Antifolate Super-Resistance, Uganda, Tanzania, and Ethiopia.
Authors:
Alifrangis, Michael; Nag, Sidsel; Schousboe, Mette L; Ishengoma, Deus; Lusingu, John; Pota, Hirva; Kavishe, Reginald A; Pearce, Richard; Ord, Rosalynn; Lynch, Caroline; Dejene, Seyoum; Cox, Jonathan; Rwakimari, John; Minja, Daniel T R; Lemnge, Martha M; Roper, Cally
Journal:
Emerging Infectious Diseases
Abstract:
Super-resistant Plasmodium falciparum threatens the effectiveness of sulfadoxine-pyrimethamine in intermittent preventive treatment for malaria during pregnancy. It is characterized by the A581G Pfdhps mutation on a background of the double-mutant Pfdhps and the triple-mutant Pfdhfr. Using samples collected during 2004-2008, we investigated the evolutionary origin of the A581G mutation by characterizing microsatellite diversity flanking Pfdhps triple-mutant (437G+540E+581G) alleles from 3 locations in eastern Africa and comparing it with double-mutant (437G+540E) alleles from the same area. In Ethiopia, both alleles derived from 1 lineage that was distinct from those in Uganda and Tanzania. Uganda and Tanzania triple mutants derived from the previously characterized southeastern Africa double-mutant lineage. The A581G mutation has occurred multiple times on local Pfdhps double-mutant backgrounds; however, a novel microsatellite allele incorporated into the Tanzania lineage since 2004 illustrates the local expansion of emergent triple-mutant lineages.
Issue Date:
Aug-2014
URI:
http://hdl.handle.net/10144/325834
DOI:
10.3201/eid2008.131897
PubMed ID:
25061906
Language:
en
ISSN:
1080-6059
Appears in Collections:
Malaria

Full metadata record

DC FieldValue Language
dc.contributor.authorAlifrangis, Michaelen_GB
dc.contributor.authorNag, Sidselen_GB
dc.contributor.authorSchousboe, Mette Len_GB
dc.contributor.authorIshengoma, Deusen_GB
dc.contributor.authorLusingu, Johnen_GB
dc.contributor.authorPota, Hirvaen_GB
dc.contributor.authorKavishe, Reginald Aen_GB
dc.contributor.authorPearce, Richarden_GB
dc.contributor.authorOrd, Rosalynnen_GB
dc.contributor.authorLynch, Carolineen_GB
dc.contributor.authorDejene, Seyoumen_GB
dc.contributor.authorCox, Jonathanen_GB
dc.contributor.authorRwakimari, Johnen_GB
dc.contributor.authorMinja, Daniel T Ren_GB
dc.contributor.authorLemnge, Martha Men_GB
dc.contributor.authorRoper, Callyen_GB
dc.date.accessioned2014-09-03T18:57:17Z-
dc.date.available2014-09-03T18:57:17Z-
dc.date.issued2014-08-
dc.identifier.citationIndependent Origin of Plasmodium falciparum Antifolate Super-Resistance, Uganda, Tanzania, and Ethiopia. 2014, 20 (8):1280-6 Emerging Infect. Dis.en_GB
dc.identifier.issn1080-6059-
dc.identifier.pmid25061906-
dc.identifier.doi10.3201/eid2008.131897-
dc.identifier.urihttp://hdl.handle.net/10144/325834-
dc.description.abstractSuper-resistant Plasmodium falciparum threatens the effectiveness of sulfadoxine-pyrimethamine in intermittent preventive treatment for malaria during pregnancy. It is characterized by the A581G Pfdhps mutation on a background of the double-mutant Pfdhps and the triple-mutant Pfdhfr. Using samples collected during 2004-2008, we investigated the evolutionary origin of the A581G mutation by characterizing microsatellite diversity flanking Pfdhps triple-mutant (437G+540E+581G) alleles from 3 locations in eastern Africa and comparing it with double-mutant (437G+540E) alleles from the same area. In Ethiopia, both alleles derived from 1 lineage that was distinct from those in Uganda and Tanzania. Uganda and Tanzania triple mutants derived from the previously characterized southeastern Africa double-mutant lineage. The A581G mutation has occurred multiple times on local Pfdhps double-mutant backgrounds; however, a novel microsatellite allele incorporated into the Tanzania lineage since 2004 illustrates the local expansion of emergent triple-mutant lineages.en_GB
dc.language.isoenen
dc.rightsPublished by Centers for Disease Control (CDC) Archived on this site by permission of CDC, [url]http://www.cdc.gov/ncidod/eid[/url]en_GB
dc.titleIndependent Origin of Plasmodium falciparum Antifolate Super-Resistance, Uganda, Tanzania, and Ethiopia.en
dc.identifier.journalEmerging Infectious Diseasesen_GB

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