Decontamination methods for samples preserved in cetylpyridinium chloride and cultured on thin-layer agar.

Hdl Handle:
http://hdl.handle.net/10144/326068
Title:
Decontamination methods for samples preserved in cetylpyridinium chloride and cultured on thin-layer agar.
Authors:
Ardizzoni, E; Mulders, W; Sanchez-Padilla, E; Varaine, F; de Jong, B C; Rigouts, L
Journal:
The International Journal of Tuberculosis and Lung Disease : the official journal of the International Union against Tuberculosis and Lung Disease
Abstract:
Long transportation times of samples to culture laboratories can lead to higher contamination rates and significant loss of viability, resulting in lower culture positivity rates. Thin-layer agar (TLA) is a sensitive culture method for the isolation of Mycobacterium tuberculosis that has been optimised with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) decontaminated samples. The combination of the TLA culture method and other decontamination procedures has not been extensively validated.
Issue Date:
1-Aug-2014
URI:
http://hdl.handle.net/10144/326068
DOI:
10.5588/ijtld.13.0887
PubMed ID:
25199014
Language:
en
ISSN:
1815-7920
Appears in Collections:
Laboratory

Full metadata record

DC FieldValue Language
dc.contributor.authorArdizzoni, Een_GB
dc.contributor.authorMulders, Wen_GB
dc.contributor.authorSanchez-Padilla, Een_GB
dc.contributor.authorVaraine, Fen_GB
dc.contributor.authorde Jong, B Cen_GB
dc.contributor.authorRigouts, Len_GB
dc.date.accessioned2014-09-11T21:37:53Z-
dc.date.available2014-09-11T21:37:53Z-
dc.date.issued2014-08-01-
dc.identifier.citationDecontamination methods for samples preserved in cetylpyridinium chloride and cultured on thin-layer agar. 2014, 18 (8):972-7 Int. J. Tuberc. Lung Dis.en_GB
dc.identifier.issn1815-7920-
dc.identifier.pmid25199014-
dc.identifier.doi10.5588/ijtld.13.0887-
dc.identifier.urihttp://hdl.handle.net/10144/326068-
dc.description.abstractLong transportation times of samples to culture laboratories can lead to higher contamination rates and significant loss of viability, resulting in lower culture positivity rates. Thin-layer agar (TLA) is a sensitive culture method for the isolation of Mycobacterium tuberculosis that has been optimised with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) decontaminated samples. The combination of the TLA culture method and other decontamination procedures has not been extensively validated.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to The International Journal of Tuberculosis and Lung Disease : the official journal of the International Union against Tuberculosis and Lung Diseaseen_GB
dc.titleDecontamination methods for samples preserved in cetylpyridinium chloride and cultured on thin-layer agar.en
dc.identifier.journalThe International Journal of Tuberculosis and Lung Disease : the official journal of the International Union against Tuberculosis and Lung Diseaseen_GB
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