Malaria PCR Detection in Cambodian Low-Transmission Settings: Dried Blood Spots Versus Venous Blood Samples

Hdl Handle:
http://hdl.handle.net/10144/344014
Title:
Malaria PCR Detection in Cambodian Low-Transmission Settings: Dried Blood Spots Versus Venous Blood Samples
Authors:
Canier, Lydie; Khim, Nimol; Kim, Saorin; Eam, Rotha; Khean, Chanra; Loch, Kaknika; Ken, Malen; Pannus, Pieter; Bosman, Philippe; Stassijns, Jorgen; Nackers, Fabienne; Alipon, SweetC; Char, Meng Chuor; Chea, Nguon; Etienne, William; De Smet, Martin; Kindermans, Jean-Marie; Ménard, Didier
Journal:
The American Journal of Tropical Medicine and Hygiene
Abstract:
In the context of malaria elimination, novel strategies for detecting very low malaria parasite densities in asymptomatic individuals are needed. One of the major limitations of the malaria parasite detection methods is the volume of blood samples being analyzed. The objective of the study was to compare the diagnostic accuracy of a malaria polymerase chain reaction assay, from dried blood spots (DBS, 5 μL) and different volumes of venous blood (50 μL, 200 μL, and 1 mL). The limit of detection of the polymerase chain reaction assay, using calibrated Plasmodium falciparum blood dilutions, showed that venous blood samples (50 μL, 200 μL, 1 mL) combined with Qiagen extraction methods gave a similar threshold of 100 parasites/mL, ∼100-fold lower than 5 μL DBS/Instagene method. On a set of 521 field samples, collected in two different transmission areas in northern Cambodia, no significant difference in the proportion of parasite carriers, regardless of the methods used was found. The 5 μL DBS method missed 27% of the samples detected by the 1 mL venous blood method, but most of the missed parasites carriers were infected by Plasmodium vivax (84%). The remaining missed P. falciparum parasite carriers (N = 3) were only detected in high-transmission areas.
Publisher:
American Society of Tropical Medicine and Hygiene
Issue Date:
5-Jan-2015
URI:
http://hdl.handle.net/10144/344014
DOI:
10.4269/ajtmh.14-0614
PubMed ID:
25561570
Additional Links:
http://www.ajtmh.org
Language:
en
ISSN:
1476-1645
Appears in Collections:
Malaria

Full metadata record

DC FieldValue Language
dc.contributor.authorCanier, Lydieen_GB
dc.contributor.authorKhim, Nimolen_GB
dc.contributor.authorKim, Saorinen_GB
dc.contributor.authorEam, Rothaen_GB
dc.contributor.authorKhean, Chanraen_GB
dc.contributor.authorLoch, Kaknikaen_GB
dc.contributor.authorKen, Malenen_GB
dc.contributor.authorPannus, Pieteren_GB
dc.contributor.authorBosman, Philippeen_GB
dc.contributor.authorStassijns, Jorgenen_GB
dc.contributor.authorNackers, Fabienneen_GB
dc.contributor.authorAlipon, SweetCen_GB
dc.contributor.authorChar, Meng Chuoren_GB
dc.contributor.authorChea, Nguonen_GB
dc.contributor.authorEtienne, Williamen_GB
dc.contributor.authorDe Smet, Martinen_GB
dc.contributor.authorKindermans, Jean-Marieen_GB
dc.contributor.authorMénard, Didieren_GB
dc.date.accessioned2015-01-30T16:15:17Z-
dc.date.available2015-01-30T16:15:17Z-
dc.date.issued2015-01-05-
dc.identifier.citationMalaria PCR Detection in Cambodian Low-Transmission Settings: Dried Blood Spots Versus Venous Blood Samples. 2015: Am. J. Trop. Med. Hyg.en_GB
dc.identifier.issn1476-1645-
dc.identifier.pmid25561570-
dc.identifier.doi10.4269/ajtmh.14-0614-
dc.identifier.urihttp://hdl.handle.net/10144/344014-
dc.description.abstractIn the context of malaria elimination, novel strategies for detecting very low malaria parasite densities in asymptomatic individuals are needed. One of the major limitations of the malaria parasite detection methods is the volume of blood samples being analyzed. The objective of the study was to compare the diagnostic accuracy of a malaria polymerase chain reaction assay, from dried blood spots (DBS, 5 μL) and different volumes of venous blood (50 μL, 200 μL, and 1 mL). The limit of detection of the polymerase chain reaction assay, using calibrated Plasmodium falciparum blood dilutions, showed that venous blood samples (50 μL, 200 μL, 1 mL) combined with Qiagen extraction methods gave a similar threshold of 100 parasites/mL, ∼100-fold lower than 5 μL DBS/Instagene method. On a set of 521 field samples, collected in two different transmission areas in northern Cambodia, no significant difference in the proportion of parasite carriers, regardless of the methods used was found. The 5 μL DBS method missed 27% of the samples detected by the 1 mL venous blood method, but most of the missed parasites carriers were infected by Plasmodium vivax (84%). The remaining missed P. falciparum parasite carriers (N = 3) were only detected in high-transmission areas.en_GB
dc.languageENG-
dc.language.isoenen
dc.publisherAmerican Society of Tropical Medicine and Hygieneen_GB
dc.relation.urlhttp://www.ajtmh.orgen_GB
dc.rightsArchived with thanks to The American Journal of Tropical Medicine and Hygieneen_GB
dc.titleMalaria PCR Detection in Cambodian Low-Transmission Settings: Dried Blood Spots Versus Venous Blood Samplesen
dc.identifier.journalThe American Journal of Tropical Medicine and Hygieneen_GB
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