Rapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system.

Hdl Handle:
http://hdl.handle.net/10144/48815
Title:
Rapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system.
Authors:
Martin, A; von Groll, A; Fissette, K; Palomino, J C; Varaine, F; Portaels, F
Journal:
Journal of Clinical Microbiology
Abstract:
The objective of this study was to evaluate the manual mycobacterium growth indicator tube (MGIT) system for the testing of Mycobacterium tuberculosis susceptibility to second-line drugs compared to the proportion method. One hundred eighty-eight M. tuberculosis isolates were tested for susceptibility to ofloxacin, kanamycin, ethionamide, and capreomycin by the manual MGIT, and results were compared to those obtained with the proportion method on 7H11 agar, considered a reference method. Results for ofloxacin and capreomycin were excellent, with 100% accuracy, and a result of 99.4% accuracy was achieved for kanamycin. For ethionamide, accuracy was lower, with a result of 86.7% compared to that of the proportion method. We proposed the following critical concentrations for the drugs: for ofloxacin, 2.0 microg/ml; for kanamycin, 2.5 microg/ml; for ethionamide, 5 microg/ml; and for capreomycin, 2.5 microg/ml. The time required to obtain results was an average of 8 days by the manual MGIT and 3 weeks by the reference method. Our results show that the manual MGIT is an accurate method for the rapid susceptibility testing of M. tuberculosis to second-line drugs. There is no need for a machine when using the manual MGIT, and results can be read with a simple UV lamp or with a semiquantitative reader, which considerably reduces the cost of the method.
Affiliation:
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium. amartin@itg.be
Issue Date:
Dec-2008
URI:
http://hdl.handle.net/10144/48815
DOI:
10.1128/JCM.01171-08
PubMed ID:
18945838
Language:
en
ISSN:
1098-660X
Appears in Collections:
TB

Full metadata record

DC FieldValue Language
dc.contributor.authorMartin, A-
dc.contributor.authorvon Groll, A-
dc.contributor.authorFissette, K-
dc.contributor.authorPalomino, J C-
dc.contributor.authorVaraine, F-
dc.contributor.authorPortaels, F-
dc.date.accessioned2009-02-10T11:39:55Z-
dc.date.available2009-02-10T11:39:55Z-
dc.date.issued2008-12-
dc.identifier.citationRapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system. 2008, 46 (12):3952-6 J. Clin. Microbiol.en
dc.identifier.issn1098-660X-
dc.identifier.pmid18945838-
dc.identifier.doi10.1128/JCM.01171-08-
dc.identifier.urihttp://hdl.handle.net/10144/48815-
dc.description.abstractThe objective of this study was to evaluate the manual mycobacterium growth indicator tube (MGIT) system for the testing of Mycobacterium tuberculosis susceptibility to second-line drugs compared to the proportion method. One hundred eighty-eight M. tuberculosis isolates were tested for susceptibility to ofloxacin, kanamycin, ethionamide, and capreomycin by the manual MGIT, and results were compared to those obtained with the proportion method on 7H11 agar, considered a reference method. Results for ofloxacin and capreomycin were excellent, with 100% accuracy, and a result of 99.4% accuracy was achieved for kanamycin. For ethionamide, accuracy was lower, with a result of 86.7% compared to that of the proportion method. We proposed the following critical concentrations for the drugs: for ofloxacin, 2.0 microg/ml; for kanamycin, 2.5 microg/ml; for ethionamide, 5 microg/ml; and for capreomycin, 2.5 microg/ml. The time required to obtain results was an average of 8 days by the manual MGIT and 3 weeks by the reference method. Our results show that the manual MGIT is an accurate method for the rapid susceptibility testing of M. tuberculosis to second-line drugs. There is no need for a machine when using the manual MGIT, and results can be read with a simple UV lamp or with a semiquantitative reader, which considerably reduces the cost of the method.en
dc.language.isoenen
dc.rightsPublished by the American Society for Microbiology - Archived on this site with kind permission from the American Society for Microbiologyen
dc.subject.meshAntitubercular Agentsen
dc.subject.meshHumansen
dc.subject.meshMicrobial Sensitivity Testsen
dc.subject.meshMycobacterium tuberculosisen
dc.subject.meshSensitivity and Specificityen
dc.subject.meshTime Factorsen
dc.titleRapid detection of Mycobacterium tuberculosis resistance to second-line drugs by use of the manual mycobacterium growth indicator tube system.en
dc.contributor.departmentMycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium. amartin@itg.been
dc.identifier.journalJournal of Clinical Microbiologyen
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