Field Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greece

Hdl Handle:
http://hdl.handle.net/10144/550439
Title:
Field Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greece
Authors:
Tseroni, Maria; Pervanidou, Danai; Tserkezou, Persefoni; Rachiotis, George; Pinaka, Ourania; Baka, Agoritsa; Georgakopoulou, Theano; Vakali, Annita; Dionysopoulou, Martha; Terzaki, Irene; Marka, Andriani; Detsis, Marios; Evlampidou, Zafiroula; Mpimpa, Anastasia; Vassalou, Evdokia; Tsiodras, Sotirios; Tsakris, Athanasios; Kremastinou, Jenny; Hadjichristodoulou, Christos
Journal:
PloS One
Abstract:
Greece, a malaria-free country since 1974, has experienced re-emergence of Plasmodium vivax autochthonous malaria cases in some agriculture areas over the last three years. In early 2012, an integrated control programme (MALWEST Project) was launched in order to prevent re-establishment of the disease. In the context of this project, the rapid diagnostic tests (RDT) of SD Bioline Malaria Ag Pf/Pan that detects hrp-2 and pan-LDH antigens were used. The aim of this study was to assess the field application of the RDT for the P. vivax diagnosis in comparison to light microscopy and polymerase chain reaction (PCR). A total of 955 samples were tested with all three diagnostic tools. Agreement of RDT against microscopy and PCR for the diagnosis of P. vivax was satisfactory (K value: 0.849 and 0.976, respectively). The sensitivity, specificity and positive predictive value of RDT against PCR was 95.6% (95% C.I.: 84.8-99.3), 100% (95% C.I.: 99.6-100.0) and 100% (95% CI: 91.7-100.0) respectively, while the sensitivity, specificity and positive predictive value of RDT against microscopic examination was 97.4% (95% C.I.: 86.1-99.6), 99.4% (95% C.I.: 98.6-99.8) and 86.1% (95% CI: 72.1-94.7), respectively. Our results indicate that RDT performed satisfactory in a non-endemic country and therefore is recommended for malaria diagnosis, especially in areas where health professionals lack experience on light microscopy.
Publisher:
Public Library of Science
Issue Date:
24-Mar-2015
URI:
http://hdl.handle.net/10144/550439
DOI:
10.1371/journal.pone.0120367
PubMed ID:
25803815
Language:
en
ISSN:
1932-6203
Appears in Collections:
Malaria

Full metadata record

DC FieldValue Language
dc.contributor.authorTseroni, Mariaen_GB
dc.contributor.authorPervanidou, Danaien_GB
dc.contributor.authorTserkezou, Persefonien_GB
dc.contributor.authorRachiotis, Georgeen_GB
dc.contributor.authorPinaka, Ouraniaen_GB
dc.contributor.authorBaka, Agoritsaen_GB
dc.contributor.authorGeorgakopoulou, Theanoen_GB
dc.contributor.authorVakali, Annitaen_GB
dc.contributor.authorDionysopoulou, Marthaen_GB
dc.contributor.authorTerzaki, Ireneen_GB
dc.contributor.authorMarka, Andrianien_GB
dc.contributor.authorDetsis, Mariosen_GB
dc.contributor.authorEvlampidou, Zafiroulaen_GB
dc.contributor.authorMpimpa, Anastasiaen_GB
dc.contributor.authorVassalou, Evdokiaen_GB
dc.contributor.authorTsiodras, Sotiriosen_GB
dc.contributor.authorTsakris, Athanasiosen_GB
dc.contributor.authorKremastinou, Jennyen_GB
dc.contributor.authorHadjichristodoulou, Christosen_GB
dc.date.accessioned2015-04-21T18:27:40Z-
dc.date.available2015-04-21T18:27:40Z-
dc.date.issued2015-03-24-
dc.identifier.citationField Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greece. 2015, 10 (3):e0120367 PLoS ONEen_GB
dc.identifier.issn1932-6203-
dc.identifier.pmid25803815-
dc.identifier.doi10.1371/journal.pone.0120367-
dc.identifier.urihttp://hdl.handle.net/10144/550439-
dc.description.abstractGreece, a malaria-free country since 1974, has experienced re-emergence of Plasmodium vivax autochthonous malaria cases in some agriculture areas over the last three years. In early 2012, an integrated control programme (MALWEST Project) was launched in order to prevent re-establishment of the disease. In the context of this project, the rapid diagnostic tests (RDT) of SD Bioline Malaria Ag Pf/Pan that detects hrp-2 and pan-LDH antigens were used. The aim of this study was to assess the field application of the RDT for the P. vivax diagnosis in comparison to light microscopy and polymerase chain reaction (PCR). A total of 955 samples were tested with all three diagnostic tools. Agreement of RDT against microscopy and PCR for the diagnosis of P. vivax was satisfactory (K value: 0.849 and 0.976, respectively). The sensitivity, specificity and positive predictive value of RDT against PCR was 95.6% (95% C.I.: 84.8-99.3), 100% (95% C.I.: 99.6-100.0) and 100% (95% CI: 91.7-100.0) respectively, while the sensitivity, specificity and positive predictive value of RDT against microscopic examination was 97.4% (95% C.I.: 86.1-99.6), 99.4% (95% C.I.: 98.6-99.8) and 86.1% (95% CI: 72.1-94.7), respectively. Our results indicate that RDT performed satisfactory in a non-endemic country and therefore is recommended for malaria diagnosis, especially in areas where health professionals lack experience on light microscopy.en_GB
dc.language.isoenen
dc.publisherPublic Library of Scienceen_GB
dc.rightsPublished by Public Library of Science, [url]http://www.plosone.org/[/url] Archived on this site by Open Access permissionen_GB
dc.titleField Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greeceen
dc.identifier.journalPloS Oneen_GB

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