Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone

Hdl Handle:
http://hdl.handle.net/10144/575207
Title:
Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone
Authors:
Park, Daniel J; Dudas, Gytis; Wohl, Shirlee; Goba, Augustine; Whitmer, Shannon L M; Andersen, Kristian G; Sealfon, Rachel S; Ladner, Jason T; Kugelman, Jeffrey R; Matranga, Christian B; Winnicki, Sarah M; Qu, James; Gire, Stephen K; Gladden-Young, Adrianne; Jalloh, Simbirie; Nosamiefan, Dolo; Yozwiak, Nathan L; Moses, Lina M; Jiang, Pan-Pan; Lin, Aaron E; Schaffner, Stephen F; Bird, Brian; Towner, Jonathan; Mamoh, Mambu; Gbakie, Michael; Kanneh, Lansana; Kargbo, David; Massally, James L B; Kamara, Fatima K; Konuwa, Edwin; Sellu, Josephine; Jalloh, Abdul A; Mustapha, Ibrahim; Foday, Momoh; Yillah, Mohamed; Erickson, Bobbie R; Sealy, Tara; Blau, Dianna; Paddock, Christopher; Brault, Aaron; Amman, Brian; Basile, Jane; Bearden, Scott; Belser, Jessica; Bergeron, Eric; Campbell, Shelley; Chakrabarti, Ayan; Dodd, Kimberly; Flint, Mike; Gibbons, Aridth; Goodman, Christin; Klena, John; McMullan, Laura; Morgan, Laura; Russell, Brandy; Salzer, Johanna; Sanchez, Angela; Wang, David; Jungreis, Irwin; Tomkins-Tinch, Christopher; Kislyuk, Andrey; Lin, Michael F; Chapman, Sinead; MacInnis, Bronwyn; Matthews, Ashley; Bochicchio, James; Hensley, Lisa E; Kuhn, Jens H; Nusbaum, Chad; Schieffelin, John S; Birren, Bruce W; Forget, Marc; Nichol, Stuart T; Palacios, Gustavo F; Ndiaye, Daouda; Happi, Christian; Gevao, Sahr M; Vandi, Mohamed A; Kargbo, Brima; Holmes, Edward C; Bedford, Trevor; Gnirke, Andreas; Ströher, Ute; Rambaut, Andrew; Garry, Robert F; Sabeti, Pardis C
Journal:
Cell
Abstract:
The 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.
Issue Date:
18-Jun-2015
URI:
http://hdl.handle.net/10144/575207
DOI:
10.1016/j.cell.2015.06.007
PubMed ID:
26091036
Language:
en
ISSN:
1097-4172
Appears in Collections:
Other Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorPark, Daniel Jen_GB
dc.contributor.authorDudas, Gytisen_GB
dc.contributor.authorWohl, Shirleeen_GB
dc.contributor.authorGoba, Augustineen_GB
dc.contributor.authorWhitmer, Shannon L Men_GB
dc.contributor.authorAndersen, Kristian Gen_GB
dc.contributor.authorSealfon, Rachel Sen_GB
dc.contributor.authorLadner, Jason Ten_GB
dc.contributor.authorKugelman, Jeffrey Ren_GB
dc.contributor.authorMatranga, Christian Ben_GB
dc.contributor.authorWinnicki, Sarah Men_GB
dc.contributor.authorQu, Jamesen_GB
dc.contributor.authorGire, Stephen Ken_GB
dc.contributor.authorGladden-Young, Adrianneen_GB
dc.contributor.authorJalloh, Simbirieen_GB
dc.contributor.authorNosamiefan, Doloen_GB
dc.contributor.authorYozwiak, Nathan Len_GB
dc.contributor.authorMoses, Lina Men_GB
dc.contributor.authorJiang, Pan-Panen_GB
dc.contributor.authorLin, Aaron Een_GB
dc.contributor.authorSchaffner, Stephen Fen_GB
dc.contributor.authorBird, Brianen_GB
dc.contributor.authorTowner, Jonathanen_GB
dc.contributor.authorMamoh, Mambuen_GB
dc.contributor.authorGbakie, Michaelen_GB
dc.contributor.authorKanneh, Lansanaen_GB
dc.contributor.authorKargbo, Daviden_GB
dc.contributor.authorMassally, James L Ben_GB
dc.contributor.authorKamara, Fatima Ken_GB
dc.contributor.authorKonuwa, Edwinen_GB
dc.contributor.authorSellu, Josephineen_GB
dc.contributor.authorJalloh, Abdul Aen_GB
dc.contributor.authorMustapha, Ibrahimen_GB
dc.contributor.authorFoday, Momohen_GB
dc.contributor.authorYillah, Mohameden_GB
dc.contributor.authorErickson, Bobbie Ren_GB
dc.contributor.authorSealy, Taraen_GB
dc.contributor.authorBlau, Diannaen_GB
dc.contributor.authorPaddock, Christopheren_GB
dc.contributor.authorBrault, Aaronen_GB
dc.contributor.authorAmman, Brianen_GB
dc.contributor.authorBasile, Janeen_GB
dc.contributor.authorBearden, Scotten_GB
dc.contributor.authorBelser, Jessicaen_GB
dc.contributor.authorBergeron, Ericen_GB
dc.contributor.authorCampbell, Shelleyen_GB
dc.contributor.authorChakrabarti, Ayanen_GB
dc.contributor.authorDodd, Kimberlyen_GB
dc.contributor.authorFlint, Mikeen_GB
dc.contributor.authorGibbons, Aridthen_GB
dc.contributor.authorGoodman, Christinen_GB
dc.contributor.authorKlena, Johnen_GB
dc.contributor.authorMcMullan, Lauraen_GB
dc.contributor.authorMorgan, Lauraen_GB
dc.contributor.authorRussell, Brandyen_GB
dc.contributor.authorSalzer, Johannaen_GB
dc.contributor.authorSanchez, Angelaen_GB
dc.contributor.authorWang, Daviden_GB
dc.contributor.authorJungreis, Irwinen_GB
dc.contributor.authorTomkins-Tinch, Christopheren_GB
dc.contributor.authorKislyuk, Andreyen_GB
dc.contributor.authorLin, Michael Fen_GB
dc.contributor.authorChapman, Sineaden_GB
dc.contributor.authorMacInnis, Bronwynen_GB
dc.contributor.authorMatthews, Ashleyen_GB
dc.contributor.authorBochicchio, Jamesen_GB
dc.contributor.authorHensley, Lisa Een_GB
dc.contributor.authorKuhn, Jens Hen_GB
dc.contributor.authorNusbaum, Chaden_GB
dc.contributor.authorSchieffelin, John Sen_GB
dc.contributor.authorBirren, Bruce Wen_GB
dc.contributor.authorForget, Marcen_GB
dc.contributor.authorNichol, Stuart Ten_GB
dc.contributor.authorPalacios, Gustavo Fen_GB
dc.contributor.authorNdiaye, Daoudaen_GB
dc.contributor.authorHappi, Christianen_GB
dc.contributor.authorGevao, Sahr Men_GB
dc.contributor.authorVandi, Mohamed Aen_GB
dc.contributor.authorKargbo, Brimaen_GB
dc.contributor.authorHolmes, Edward Cen_GB
dc.contributor.authorBedford, Trevoren_GB
dc.contributor.authorGnirke, Andreasen_GB
dc.contributor.authorStröher, Uteen_GB
dc.contributor.authorRambaut, Andrewen_GB
dc.contributor.authorGarry, Robert Fen_GB
dc.contributor.authorSabeti, Pardis Cen_GB
dc.date.accessioned2015-08-18T21:27:33Z-
dc.date.available2015-08-18T21:27:33Z-
dc.date.issued2015-06-18-
dc.identifier.citationEbola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone. 2015, 161 (7):1516-26 Cellen_GB
dc.identifier.issn1097-4172-
dc.identifier.pmid26091036-
dc.identifier.doi10.1016/j.cell.2015.06.007-
dc.identifier.urihttp://hdl.handle.net/10144/575207-
dc.description.abstractThe 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.en_GB
dc.language.isoenen
dc.rightsArchived with thanks to Cell's open accessen_GB
dc.titleEbola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leoneen
dc.identifier.journalCellen_GB

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