Cholera Rapid Test with Enrichment Step Has Diagnostic Performance Equivalent to Culture

Hdl Handle:
http://hdl.handle.net/10144/618744
Title:
Cholera Rapid Test with Enrichment Step Has Diagnostic Performance Equivalent to Culture
Authors:
Ontweka, LN; Deng, LO; Rauzier, J; Debes, AK; Tadesse, F; Parker, LA; Wamala, JF; Bior, BK; Lasuba, M; But, AB; Grandesso, F; Jamet, C; Cohuet, S; Ciglenecki, I; Serafini, M; Sack, DA; Quilici, ML; Azman, AS; Luquero, FJ; Page, AL
Journal:
PloS One
Abstract:
Cholera rapid diagnostic tests (RDT) could play a central role in outbreak detection and surveillance in low-resource settings, but their modest performance has hindered their broad adoption. The addition of an enrichment step may improve test specificity. We describe the results of a prospective diagnostic evaluation of the Crystal VC RDT (Span Diagnostics, India) with enrichment step and of culture, each compared to polymerase chain reaction (PCR), during a cholera outbreak in South Sudan. RDTs were performed on alkaline peptone water inoculated with stool and incubated for 4-6 hours at ambient temperature. Cholera culture was performed from wet filter paper inoculated with stool. Molecular detection of Vibrio cholerae O1 by PCR was done from dry Whatman 903 filter papers inoculated with stool, and from wet filter paper supernatant. In August and September 2015, 101 consecutive suspected cholera cases were enrolled, of which 36 were confirmed by PCR. The enriched RDT had 86.1% (95% CI: 70.5-95.3) sensitivity and 100% (95% CI: 94.4-100) specificity compared to PCR as the reference standard. The sensitivity of culture versus PCR was 83.3% (95% CI: 67.2-93.6) for culture performed on site and 72.2% (95% CI: 54.8-85.8) at the international reference laboratory, where samples were tested after an average delay of two months after sample collection, and specificity was 98.5% (95% CI: 91.7-100) and 100% (95% CI: 94.5-100), respectively. The RDT with enrichment showed performance comparable to that of culture and could be a sustainable alternative to culture confirmation where laboratory capacity is limited.
Publisher:
Public Library of Science
Issue Date:
19-Dec-2016
URI:
http://hdl.handle.net/10144/618744
DOI:
10.1371/journal.pone.0168257
PubMed ID:
27992488
Submitted date:
2017-01-11
Language:
en
ISSN:
1932-6203
Appears in Collections:
Other Diseases

Full metadata record

DC FieldValue Language
dc.contributor.authorOntweka, LNen
dc.contributor.authorDeng, LOen
dc.contributor.authorRauzier, Jen
dc.contributor.authorDebes, AKen
dc.contributor.authorTadesse, Fen
dc.contributor.authorParker, LAen
dc.contributor.authorWamala, JFen
dc.contributor.authorBior, BKen
dc.contributor.authorLasuba, Men
dc.contributor.authorBut, ABen
dc.contributor.authorGrandesso, Fen
dc.contributor.authorJamet, Cen
dc.contributor.authorCohuet, Sen
dc.contributor.authorCiglenecki, Ien
dc.contributor.authorSerafini, Men
dc.contributor.authorSack, DAen
dc.contributor.authorQuilici, MLen
dc.contributor.authorAzman, ASen
dc.contributor.authorLuquero, FJen
dc.contributor.authorPage, ALen
dc.date.accessioned2017-01-12T20:57:14Z-
dc.date.available2017-01-12T20:57:14Z-
dc.date.issued2016-12-19-
dc.date.submitted2017-01-11-
dc.identifier.citationCholera Rapid Test with Enrichment Step Has Diagnostic Performance Equivalent to Culture. 2016, 11 (12):e0168257 PLoS ONEen
dc.identifier.issn1932-6203-
dc.identifier.pmid27992488-
dc.identifier.doi10.1371/journal.pone.0168257-
dc.identifier.urihttp://hdl.handle.net/10144/618744-
dc.description.abstractCholera rapid diagnostic tests (RDT) could play a central role in outbreak detection and surveillance in low-resource settings, but their modest performance has hindered their broad adoption. The addition of an enrichment step may improve test specificity. We describe the results of a prospective diagnostic evaluation of the Crystal VC RDT (Span Diagnostics, India) with enrichment step and of culture, each compared to polymerase chain reaction (PCR), during a cholera outbreak in South Sudan. RDTs were performed on alkaline peptone water inoculated with stool and incubated for 4-6 hours at ambient temperature. Cholera culture was performed from wet filter paper inoculated with stool. Molecular detection of Vibrio cholerae O1 by PCR was done from dry Whatman 903 filter papers inoculated with stool, and from wet filter paper supernatant. In August and September 2015, 101 consecutive suspected cholera cases were enrolled, of which 36 were confirmed by PCR. The enriched RDT had 86.1% (95% CI: 70.5-95.3) sensitivity and 100% (95% CI: 94.4-100) specificity compared to PCR as the reference standard. The sensitivity of culture versus PCR was 83.3% (95% CI: 67.2-93.6) for culture performed on site and 72.2% (95% CI: 54.8-85.8) at the international reference laboratory, where samples were tested after an average delay of two months after sample collection, and specificity was 98.5% (95% CI: 91.7-100) and 100% (95% CI: 94.5-100), respectively. The RDT with enrichment showed performance comparable to that of culture and could be a sustainable alternative to culture confirmation where laboratory capacity is limited.en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rightsPublished by Public Library of Science, [url]http://www.plosone.org/[/url] Archived on this site by Open Access permissionen
dc.titleCholera Rapid Test with Enrichment Step Has Diagnostic Performance Equivalent to Cultureen
dc.identifier.journalPloS Oneen

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