Dried blood spots are a useful tool for quality assurance of rapid HIV testing in Kigali, Rwanda.

Hdl Handle:
http://hdl.handle.net/10144/71897
Title:
Dried blood spots are a useful tool for quality assurance of rapid HIV testing in Kigali, Rwanda.
Authors:
Chaillet, P; Zachariah, R; Harries, K; Rusanganwa, E; Harries, A D
Journal:
Transactions of the Royal Society of Tropical Medicine and Hygiene
Abstract:
A study was conducted in two primary health facilities in Kigali, Rwanda, to determine whether dried blood spots (DBS) used for quality control of HIV testing would give comparable results with serum after being stored for a period of 14 days and 30 days at ambient temperature. DBS and serum specimens were collected from patients undergoing HIV testing. ELISA performed on serum at baseline (gold standard) was compared with DBS results. The study included a total of 491 patients, comprising 92 (19%) males and 399 (81%) females with a median age of 27 years. A total of 148 individuals (30%) were HIV-positive. The average ambient temperature under which DBS specimens were stored at the health facilities was 23 degrees C (range 18-25 degrees C). The kappa statistic at 14 days and 30 days was 0.99 (99.4% agreement) and 0.98 (99.2% agreement), respectively, signifying almost 'perfect agreement (P<0.001)' with the gold standard. In a resource-limited sub-Saharan African country embarking on scaling-up of HIV testing, DBS stored at ambient conditions for up to 1 month were found to be a useful and robust tool to perform quality control of rapid HIV testing at the health centre level.
Affiliation:
Médecins sans Frontières, Medical Department, Brussels Operational Center, Belgium.
Publisher:
Published by Elsevier
Issue Date:
Jun-2009
URI:
http://hdl.handle.net/10144/71897
DOI:
10.1016/j.trstmh.2009.01.023
PubMed ID:
19249069
Additional Links:
http://www.sciencedirect.com/science/journal/00359203
Language:
en
ISSN:
0035-9203
Appears in Collections:
HIV/AIDS

Full metadata record

DC FieldValue Language
dc.contributor.authorChaillet, P-
dc.contributor.authorZachariah, R-
dc.contributor.authorHarries, K-
dc.contributor.authorRusanganwa, E-
dc.contributor.authorHarries, A D-
dc.date.accessioned2009-06-30T09:36:14Z-
dc.date.available2009-06-30T09:36:14Z-
dc.date.issued2009-06-
dc.identifier.citationDried blood spots are a useful tool for quality assurance of rapid HIV testing in Kigali, Rwanda. 2009, 103 (6):634-7 Trans. R. Soc. Trop. Med. Hyg.en
dc.identifier.issn0035-9203-
dc.identifier.pmid19249069-
dc.identifier.doi10.1016/j.trstmh.2009.01.023-
dc.identifier.urihttp://hdl.handle.net/10144/71897-
dc.description.abstractA study was conducted in two primary health facilities in Kigali, Rwanda, to determine whether dried blood spots (DBS) used for quality control of HIV testing would give comparable results with serum after being stored for a period of 14 days and 30 days at ambient temperature. DBS and serum specimens were collected from patients undergoing HIV testing. ELISA performed on serum at baseline (gold standard) was compared with DBS results. The study included a total of 491 patients, comprising 92 (19%) males and 399 (81%) females with a median age of 27 years. A total of 148 individuals (30%) were HIV-positive. The average ambient temperature under which DBS specimens were stored at the health facilities was 23 degrees C (range 18-25 degrees C). The kappa statistic at 14 days and 30 days was 0.99 (99.4% agreement) and 0.98 (99.2% agreement), respectively, signifying almost 'perfect agreement (P<0.001)' with the gold standard. In a resource-limited sub-Saharan African country embarking on scaling-up of HIV testing, DBS stored at ambient conditions for up to 1 month were found to be a useful and robust tool to perform quality control of rapid HIV testing at the health centre level.en
dc.language.isoenen
dc.publisherPublished by Elsevieren
dc.relation.urlhttp://www.sciencedirect.com/science/journal/00359203en
dc.rightsPublished by Elsevier Archived on this site with the kind permission of Elsevier Ltd. ([url]http://www.sciencedirect.com/science/journal/00359203[/url]) and the Royal Society of Tropical Medicine and Hygiene ([url]http://www.rstmh.org/transactions.asp[/url])en
dc.titleDried blood spots are a useful tool for quality assurance of rapid HIV testing in Kigali, Rwanda.en
dc.contributor.departmentMédecins sans Frontières, Medical Department, Brussels Operational Center, Belgium.en
dc.identifier.journalTransactions of the Royal Society of Tropical Medicine and Hygieneen

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