• Surveillance of Plasmodium falciparum pfcrt haplotypes in southwestern uganda by high-resolution melt analysis.

      Kassaza, K; Long, AC; McDaniels, JM; Andre, M; Fredrickson, W; Nyehangane, D; Orikiriza, P; Operario, DJ; Bazira, J; Mwanga-Amumpaire, JA; et al. (BMC, 2021-02-25)
      The sample source (i.e. Giemsa-stained slides or blood spots) and type of LCGreen-based reagent mixes did not impact the success of PCR-HRM. The detection limit of 10- 5 ng and the ability to identify mixed haplotypes as low as 10 % was similar to other HRM platforms. The CVIET haplotype predominated in the clinical samples (66 %, 162/244); however, there was a large regional variation between the sample groups (94 % CVIET in Group 1 and 44 % CVIET in Group 2).