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    Mar 02, 2021
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    Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates

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    Authors
    Martin, A
    Paasch, F
    Von Groll, A
    Fissette, K
    Almeida, P
    Varaine, F
    Portaels, F
    Palomino, J-C
    Affiliation
    Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium; Laboratory of Mycobacteriology, Universidade Federal do Rio Grande, Rio Grande do Sul, Brazil; Médecins Sans Frontières, Paris, France
    Issue Date
    2009-10-01
    
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    Journal
    International Journal of Tuberculosis and Lung Disease
    Abstract
    BACKGROUND: In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics. OBJECTIVE: To evaluate the thin-layer agar (TLA) for rapid detection of resistance to rifampicin (RMP), ofloxacin (OFX) and kanamycin (KM) in M. tuberculosis clinical isolates and to determine the sensitivity, specificity and time to positivity compared to the gold standard method. METHODS: One hundred and forty-seven clinical isolates of M. tuberculosis were studied. For the TLA method, a quadrant Petri plate containing 7H11 agar with RMP, OFX and KM was used. Results were compared to the Bactec MGIT960 for RMP and the proportion method for OFX and KM. RESULTS: The sensitivity and specificity for RMP and OFX were 100% and for KM they were 100% and 98.7%, respectively. The use of a TLA quadrant plate enables the rapid detection of resistance to the three anti-tuberculosis drugs RMP, OFX and KM in a median of 10 days. CONCLUSION: TLA was an accurate method for the detection of resistance in the three drugs studied. This faster method is simple to perform, providing an alternative method when more sophisticated techniques are not available in low-resource settings.
    URI
    http://hdl.handle.net/10144/116329
    PubMed ID
    19793437
    Additional Links
    http://www.ingentaconnect.com/content/iuatld/ijtld/2009/00000013/00000010/art00019?token=004b10b9383a4b3b2570232b2f5f7338432c2079662a726e2d2954496f642f466f0498d5d9d
    Type
    Article
    Language
    en
    ISSN
    1815-7920
    Collections
    TB

    entitlement

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