• Dilution Testing Using Rapid Diagnostic Tests in a HIV Diagnostic Algorithm: a Novel Alternative for Confirmation Testing in Resource Limited Settings

      Shanks, L; Siddiqui, MR; Abebe, A; Piriou, E; Pearce, N; Ariti, C; Masiga, J; Muluneh, L; Wazome, J; Ritmeijer, K; Klarkowski, D (BioMed Central, 2015-05-14)
      Current WHO testing guidelines for resource limited settings diagnose HIV on the basis of screening tests without a confirmation test due to cost constraints. This leads to a potential risk of false positive HIV diagnosis. In this paper, we evaluate the dilution test, a novel method for confirmation testing, which is simple, rapid, and low cost. The principle of the dilution test is to alter the sensitivity of a rapid diagnostic test (RDT) by dilution of the sample, in order to screen out the cross reacting antibodies responsible for falsely positive RDT results.