• Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis.

      Palomino, J C; Martin, A; Von Groll, A; Portaels, F; Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium. (Elsevier, 2008-10)
      Tuberculosis still represents a major public health problem, especially in low-resource countries where the burden of the disease is more important. Multidrug-resistant and extensively drug drug-resistant tuberculosis constitute serious problems for the efficient control of the disease stressing the need to investigate resistance to first- and second-line drugs. Conventional methods for detecting drug-resistance in Mycobacterium tuberculosis are slow and cumbersome. The most commonly used proportion method on Löwenstein-Jensen medium or Middlebrook agar requires a minimum of 3-4 weeks to produce results. Several new approaches have been proposed in the last years for the rapid and timely detection of drug-resistance in tuberculosis. This review will address phenotypic culture-based methods for rapid drug susceptibility testing in M. tuberculosis.
    • Thin layer agar compared to BACTEC MGIT 960 for early detection of Mycobacterium tuberculosis

      Martin, A; Fissette, K; Varaine, F; Portaels, F; Palomino, J C; Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium; Médecins Sans Frontières, Paris, France (2009-07-05)
      We compared the sensitivity and time to detection of growth of Mycobacterium tuberculosis in the thin layer agar (TLA) compared to BACTEC MGIT960. The average time for growth of M. tuberculosis in TLA and BACTEC MGIT960 was 10.6 and 9.6 days, respectively. The sensitivity of detection of M. tuberculosis was 97.3% on TLA and 97% on BACTEC MGIT960 for smear positive samples. TLA showed comparable results to BACTEC MGIT960 and could be an alternative method for low-income countries.