• Thin layer agar compared to BACTEC MGIT 960 for early detection of Mycobacterium tuberculosis

      Martin, A; Fissette, K; Varaine, F; Portaels, F; Palomino, J C; Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium; Médecins Sans Frontières, Paris, France (2009-07-05)
      We compared the sensitivity and time to detection of growth of Mycobacterium tuberculosis in the thin layer agar (TLA) compared to BACTEC MGIT960. The average time for growth of M. tuberculosis in TLA and BACTEC MGIT960 was 10.6 and 9.6 days, respectively. The sensitivity of detection of M. tuberculosis was 97.3% on TLA and 97% on BACTEC MGIT960 for smear positive samples. TLA showed comparable results to BACTEC MGIT960 and could be an alternative method for low-income countries.
    • Thin-layer agar for detection of resistance to rifampicin, ofloxacin and kanamycin in Mycobacterium tuberculosis isolates

      Martin, A; Paasch, F; Von Groll, A; Fissette, K; Almeida, P; Varaine, F; Portaels, F; Palomino, J-C; Institute of Tropical Medicine, Mycobacteriology Unit, Antwerp, Belgium; Laboratory of Mycobacteriology, Universidade Federal do Rio Grande, Rio Grande do Sul, Brazil; Médecins Sans Frontières, Paris, France (2009-10-01)
      BACKGROUND: In low-income countries there is a great need for economical methods for testing the susceptibility of Mycobacterium tuberculosis to antibiotics. OBJECTIVE: To evaluate the thin-layer agar (TLA) for rapid detection of resistance to rifampicin (RMP), ofloxacin (OFX) and kanamycin (KM) in M. tuberculosis clinical isolates and to determine the sensitivity, specificity and time to positivity compared to the gold standard method. METHODS: One hundred and forty-seven clinical isolates of M. tuberculosis were studied. For the TLA method, a quadrant Petri plate containing 7H11 agar with RMP, OFX and KM was used. Results were compared to the Bactec MGIT960 for RMP and the proportion method for OFX and KM. RESULTS: The sensitivity and specificity for RMP and OFX were 100% and for KM they were 100% and 98.7%, respectively. The use of a TLA quadrant plate enables the rapid detection of resistance to the three anti-tuberculosis drugs RMP, OFX and KM in a median of 10 days. CONCLUSION: TLA was an accurate method for the detection of resistance in the three drugs studied. This faster method is simple to perform, providing an alternative method when more sophisticated techniques are not available in low-resource settings.