• Login
    View Item 
    •   Home
    • 1 Published Research and Commentary
    • Other Diseases
    • View Item
    •   Home
    • 1 Published Research and Commentary
    • Other Diseases
    • View Item
    Jan 16, 2021
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of MSFTitleAuthorsSubjectsPublisherJournalThis CollectionTitleAuthorsSubjectsPublisherJournal

    Meningitis Dipstick Rapid Test: Evaluating Diagnostic Performance During an Urban Neisseria Meningitidis Serogroup A Outbreak, Burkina Faso, 2007

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Meningitis dipstick rapid test.pdf
    Size:
    278.5Kb
    Format:
    PDF
    Description:
    Main Article
    Download
    Authors
    Rose, Angela M C
    Mueller, Judith E
    Gerstl, Sibylle
    Njanpop-Lafourcade, Berthe-Marie
    Page, Anne-Laure
    Nicolas, Pierre
    Traoré, Ramata Ouédraogo
    Caugant, Dominique A
    Guerin, Philippe J
    Affiliation
    Epicentre, France; Chronic Disease Research Centre, University of the West Indies, West Indies; Agence de Medecine Preventive, France; Institut de Medecine Tropicale du Service de Sante des Armees (IMTSSA); World Health Organization Collaborating Centre for Reference and Research on Meningococci, France; Laboratoire de Biologie, Centre Hospitalier Universitaire Pediatrique Charles de Gaulle, Burkina Faso; World Health Organization, Collaborating Centre for Reference and Research on Meningococci, Norwegian Institute of Public Health, Norway; Institute of General Practice and Community Medicine, University of Oslo, Norway
    Issue Date
    2010-06-11
    Submitted date
    2011-03-10
    
    Metadata
    Show full item record
    Journal
    PloS One
    Abstract
    Meningococcal meningitis outbreaks occur every year during the dry season in the "meningitis belt" of sub-Saharan Africa. Identification of the causative strain is crucial before launching mass vaccination campaigns, to assure use of the correct vaccine. Rapid agglutination (latex) tests are most commonly available in district-level laboratories at the beginning of the epidemic season; limitations include a short shelf-life and the need for refrigeration and good technical skills. Recently, a new dipstick rapid diagnostic test (RDT) was developed to identify and differentiate disease caused by meningococcal serogroups A, W135, C and Y. We evaluated the diagnostic performance of this dipstick RDT during an urban outbreak of meningitis caused by N. meningitidis serogroup A in Ouagadougou, Burkina Faso; first against an in-country reference standard of culture and/or multiplex PCR; and second against culture and/or a highly sensitive nested PCR technique performed in Oslo, Norway. We included 267 patients with suspected acute bacterial meningitis. Using the in-country reference standard, 50 samples (19%) were positive. Dipstick RDT sensitivity (N = 265) was 70% (95%CI 55-82) and specificity 97% (95%CI 93-99). Using culture and/or nested PCR, 126/259 (49%) samples were positive; dipstick RDT sensitivity (N = 257) was 32% (95%CI 24-41), and specificity was 99% (95%CI 95-100). We found dipstick RDT sensitivity lower than values reported from (i) assessments under ideal laboratory conditions (>90%), and (ii) a prior field evaluation in Niger [89% (95%CI 80-95)]. Specificity, however, was similar to (i), and higher than (ii) [62% (95%CI 48-75)]. At this stage in development, therefore, other tests (e.g., latex) might be preferred for use in peripheral health centres. We highlight the value of field evaluations for new diagnostic tests, and note relatively low sensitivity of a reference standard using multiplex vs. nested PCR. Although the former is the current standard for bacterial meningitis surveillance in the meningitis belt, nested PCR performed in a certified laboratory should be used as an absolute reference when evaluating new diagnostic tests.
    URI
    http://hdl.handle.net/10144/127585
    DOI
    10.1371/journal.pone.0011086
    PubMed ID
    20552035
    Additional Links
    http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0011086
    Type
    Article
    Language
    en
    ISSN
    1932-6203
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0011086
    Scopus Count
    Collections
    Other Diseases

    entitlement

    Related articles

    • Field evaluation of two rapid diagnostic tests for Neisseria meningitidis serogroup A during the 2006 outbreak in Niger.
    • Authors: Rose AM, Gerstl S, Mahamane AE, Sidikou F, Djibo S, Bonte L, Caugant DA, Guerin PJ, Chanteau S
    • Issue date: 2009 Oct 5
    • New rapid diagnostic tests for Neisseria meningitidis serogroups A, W135, C, and Y.
    • Authors: Chanteau S, Dartevelle S, Mahamane AE, Djibo S, Boisier P, Nato F
    • Issue date: 2006 Sep
    • Evaluation of the Pastorex meningitis kit for the rapid identification of Neisseria meningitidis serogroups A and W135.
    • Authors: Djibo S, Njanpop Lafourcade BM, Boisier P, Moussa A, Kobo G, Sidikou F, Hien A, Bieboure G, Aguilera JF, Parent du Chatelet I, Gessner BD, Chanteau S
    • Issue date: 2006 Jun
    • Bacterial meningitis in Burkina Faso: surveillance using field-based polymerase chain reaction testing.
    • Authors: Parent du Châtelet I, Traore Y, Gessner BD, Antignac A, Naccro B, Njanpop-Lafourcade BM, Ouedraogo MS, Tiendrebeogo SR, Varon E, Taha MK
    • Issue date: 2005 Jan 1
    • A five-year field assessment of rapid diagnostic tests for meningococcal meningitis in Niger by using the combination of conventional and real-time PCR assays as a gold standard.
    • Authors: Collard JM, Wang X, Mahamane AE, Idi I, Issaka B, Ousseni M, Mayer LW, Nato F, Moulia-Pelat JP
    • Issue date: 2014 Jan
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.