• Login
    View Item 
    •   Home
    • 1 Published Research and Commentary
    • TB
    • View Item
    •   Home
    • 1 Published Research and Commentary
    • TB
    • View Item
    Apr 23, 2021
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of MSFTitleAuthorsSubjectsPublisherJournalThis CollectionTitleAuthorsSubjectsPublisherJournal

    Tuberculosis ethambutol resistance: concordance between phenotypic and genotypic test results

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    091002_Plinke_TB-ethambutol-re ...
    Size:
    157.9Kb
    Format:
    PDF
    Download
    Authors
    Plinke, C
    Cox, H S
    Kalon, S
    Doshetov, D
    Rüsch-Gerdes, S
    Niemann, S
    Affiliation
    Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany; Macfarlane Burnet Institute for Medical Research and Public Health, Australia; Me´decins Sans Frontie`res, Uzbekistan; Ministry of Health, Karakalpakstan, Uzbekistan; National Reference Center for Mycobacteria, Research Center Borstel, Borstel, Germany
    Issue Date
    2009-10-02
    
    Metadata
    Show full item record
    Journal
    Tuberculosis
    Abstract
    embB306 mutations are potential markers for detecting ethambutol resistance in clinical Mycobacterium tuberculosis isolates. However, more recently, embB306 mutations have been found in ethambutol susceptible isolates and an association with broad drug resistance rather than ethambutol resistance has been reported. To further investigate this question, we analyzed the association between embB306 mutations and phenotypic ethambutol resistance among 197 isolates from a drug resistance survey performed in Karakalpakstan, Uzbekistan. 39 strains had an embB306 mutation, out of which seven were ethambutol susceptible, thus, displaying discrepant test results. After re-analysis, the seven isolates were tested ethambutol resistant. All of these strains had an increased ethambutol MIC, however, three strains showed no or weak growth on the critical concentration of 2 microg/ml on Löwenstein-Jensen. In three strains we confirmed the presence of heteroresistant mixed populations which might influence conventional ethambutol testing. Final concordance between molecular and phenotypic EMB testing was high with a sensitivity of 78% and a specificity of 100%. Our results confirm that embB306 mutations are useful markers for predicting ethambutol resistance. Discrepancies between molecular and phenotypic ethambutol resistance test results are most likely caused by problems with conventional susceptibility testing.
    URI
    http://hdl.handle.net/10144/132830
    DOI
    10.1016/j.tube.2009.09.001
    PubMed ID
    19800845
    Additional Links
    http://www.tuberculosisjournal.com/article/S1472-9792(09)00083-3/abstract
    Type
    Article
    Language
    en
    ISSN
    1873-281X
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.tube.2009.09.001
    Scopus Count
    Collections
    TB

    entitlement

    Related articles

    • Frequency of embB codon 306 mutations in ethambutol-susceptible and -resistant clinical Mycobacterium tuberculosis isolates in Kuwait.
    • Authors: Ahmad S, Jaber AA, Mokaddas E
    • Issue date: 2007 Mar
    • Genetic characterisation of the ethambutol resistance-determining region in Mycobacterium tuberculosis: prevalence and significance of embB306 mutations.
    • Authors: Perdigão J, Macedo R, Ribeiro A, Brum L, Portugal I
    • Issue date: 2009 Apr
    • Role of embCAB gene mutations in ethambutol resistance in Mycobacterium tuberculosis isolates from India.
    • Authors: Jadaun GP, Das R, Upadhyay P, Chauhan DS, Sharma VD, Katoch VM
    • Issue date: 2009 May
    • embB306 mutations as molecular indicators to predict ethambutol susceptibility in Mycobacterium tuberculosis.
    • Authors: Sirgel FA, Warren RM, Streicher EM, Victor TC, van Helden PD, Böttger EC
    • Issue date: 2012
    • Characteristics of embB mutations in multidrug-resistant Mycobacterium tuberculosis isolates in Henan, China.
    • Authors: Shi D, Li L, Zhao Y, Jia Q, Li H, Coulter C, Jin Q, Zhu G
    • Issue date: 2011 Oct
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.