Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up.
| dc.contributor.author | Schramm, B | |
| dc.contributor.author | Hewison, C | |
| dc.contributor.author | Bonte, L | |
| dc.contributor.author | Jones, W | |
| dc.contributor.author | Camélique, O | |
| dc.contributor.author | Ruangweerayut, R | |
| dc.contributor.author | Swaddiwudhipong, W | |
| dc.contributor.author | Bonnet, M | |
| dc.date.accessioned | 2012-12-11T21:06:44Z | |
| dc.date.available | 2012-12-11T21:06:44Z | |
| dc.date.issued | 2012-08 | |
| dc.identifier.citation | J. Clin. Microbiol 2012; 50(8): 2788-2790. | en_GB |
| dc.identifier.issn | 1098-660X | |
| dc.identifier.pmid | 22649015 | |
| dc.identifier.doi | 10.1128/JCM.01232-12 | |
| dc.identifier.uri | http://hdl.handle.net/10144/255358 | |
| dc.description.abstract | Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture. | |
| dc.language.iso | en | en |
| dc.rights | Published by the American Society for Microbiology - Archived on this site with kind permission from the American Society for Microbiology | en_GB |
| dc.subject | Tuberculosis | en_GB |
| dc.title | Field evaluation of a simple fluorescence method for detection of viable Mycobacterium tuberculosis in sputum specimens during treatment follow-up. | en |
| dc.type | Article | en |
| dc.contributor.department | Epicentre, Paris, France; Médecins Sans Frontières (MSF), Paris, France;International Organization for Migration (IOM), Bangkok, Thailand; Epicentre, Geneva, Switzerlandf | en_GB |
| dc.identifier.journal | Journal of Clinical Microbiology | en_GB |
| refterms.dateFOA | 2019-03-04T10:06:29Z | |
| html.description.abstract | Simple tuberculosis (TB) treatment monitoring tools are needed. We assessed the performance of fluorescein-diacetate (FDA) smear microscopy for detection of viable Mycobacterium tuberculosis in sputum specimens (n = 288) of TB cases under treatment compared to culture (17.4% culture positivity). FDA sensitivity was moderate (83.7% [95% confidence interval {CI}, 70.3 to 92.6]), and specificity was low (66.1% [59.5 to 72.2]). The good negative predictive value (94.8% [90.1 to 97.8]) and negative likelihood ratio (0.2) suggest using this method to rule out treatment failure in settings without access to culture. |
