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dc.contributor.authorAlifrangis, Michael
dc.contributor.authorNag, Sidsel
dc.contributor.authorSchousboe, Mette L
dc.contributor.authorIshengoma, Deus
dc.contributor.authorLusingu, John
dc.contributor.authorPota, Hirva
dc.contributor.authorKavishe, Reginald A
dc.contributor.authorPearce, Richard
dc.contributor.authorOrd, Rosalynn
dc.contributor.authorLynch, Caroline
dc.contributor.authorDejene, Seyoum
dc.contributor.authorCox, Jonathan
dc.contributor.authorRwakimari, John
dc.contributor.authorMinja, Daniel T R
dc.contributor.authorLemnge, Martha M
dc.contributor.authorRoper, Cally
dc.date.accessioned2014-09-03T18:57:17Z
dc.date.available2014-09-03T18:57:17Z
dc.date.issued2014-08
dc.identifier.citationIndependent Origin of Plasmodium falciparum Antifolate Super-Resistance, Uganda, Tanzania, and Ethiopia. 2014, 20 (8):1280-6 Emerging Infect. Dis.en_GB
dc.identifier.issn1080-6059
dc.identifier.pmid25061906
dc.identifier.doi10.3201/eid2008.131897
dc.identifier.urihttp://hdl.handle.net/10144/325834
dc.description.abstractSuper-resistant Plasmodium falciparum threatens the effectiveness of sulfadoxine-pyrimethamine in intermittent preventive treatment for malaria during pregnancy. It is characterized by the A581G Pfdhps mutation on a background of the double-mutant Pfdhps and the triple-mutant Pfdhfr. Using samples collected during 2004-2008, we investigated the evolutionary origin of the A581G mutation by characterizing microsatellite diversity flanking Pfdhps triple-mutant (437G+540E+581G) alleles from 3 locations in eastern Africa and comparing it with double-mutant (437G+540E) alleles from the same area. In Ethiopia, both alleles derived from 1 lineage that was distinct from those in Uganda and Tanzania. Uganda and Tanzania triple mutants derived from the previously characterized southeastern Africa double-mutant lineage. The A581G mutation has occurred multiple times on local Pfdhps double-mutant backgrounds; however, a novel microsatellite allele incorporated into the Tanzania lineage since 2004 illustrates the local expansion of emergent triple-mutant lineages.
dc.language.isoenen
dc.rightsPublished by Centers for Disease Control (CDC) Archived on this site by permission of CDC, [url]http://www.cdc.gov/ncidod/eid[/url]en_GB
dc.titleIndependent Origin of Plasmodium falciparum Antifolate Super-Resistance, Uganda, Tanzania, and Ethiopia.en
dc.identifier.journalEmerging Infectious Diseasesen_GB
refterms.dateFOA2019-03-04T11:27:35Z
html.description.abstractSuper-resistant Plasmodium falciparum threatens the effectiveness of sulfadoxine-pyrimethamine in intermittent preventive treatment for malaria during pregnancy. It is characterized by the A581G Pfdhps mutation on a background of the double-mutant Pfdhps and the triple-mutant Pfdhfr. Using samples collected during 2004-2008, we investigated the evolutionary origin of the A581G mutation by characterizing microsatellite diversity flanking Pfdhps triple-mutant (437G+540E+581G) alleles from 3 locations in eastern Africa and comparing it with double-mutant (437G+540E) alleles from the same area. In Ethiopia, both alleles derived from 1 lineage that was distinct from those in Uganda and Tanzania. Uganda and Tanzania triple mutants derived from the previously characterized southeastern Africa double-mutant lineage. The A581G mutation has occurred multiple times on local Pfdhps double-mutant backgrounds; however, a novel microsatellite allele incorporated into the Tanzania lineage since 2004 illustrates the local expansion of emergent triple-mutant lineages.


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