SAMBA HIV semi-quantitative test, a new point-of-care viral load monitoring assay for resource-limited settings
AuthorsRitchie, Allyson V
Joshi, Hrishikesh A
De Ruiter, Annemiek
Sharma, Pia I
Lee, Helen H
MetadataShow full item record
JournalJournal of clinical microbiology
AbstractRoutine viral load (VL) testing of HIV-infected individuals on antiretroviral therapy (ART) is used to monitor treatment efficacy. However, due to logistical challenges, implementation of VL has been difficult in resource-limited settings. The aim of this study was to evaluate the performance of the SAMBA Semi-Q Test in London, Malawi, and Uganda. The SAMBA HIV-1 Semi-Q Test can distinguish between patients with VL above or below 1000 copies/ml. The SAMBA Semi-Q was validated with diluted clinical samples and blinded plasma samples collected from HIV-1-positive individuals. SAMBA Semi-Q results were compared with results from the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Test v2.0. Testing of 96 2-10 fold dilutions of four samples containing HIV-1 subtype C as well as 488 samples from patients in the United Kingdom, Malawi, and Uganda, respectively, yielded an overall accuracy for SAMBA Semi-Q of 99% (95% CI 93.8 - 99.9%) and 96.9% (95% CI 94.9 - 98.3%) respectively compared to Roche. Analysis of VL data from patients in Malawi and Uganda showed that the SAMBA cut-off of 1000 copies/ml appropriately distinguished treated from untreated individuals. Furthermore, analysis of the viral load of 232 patients on ART in Malawi and Uganda revealed similar patterns for virological control defined as either <1000 copies/ml (SAMBA cut-off) or <5000 copies/ml (WHO 2010 criterion). This study suggests that SAMBA Semi-Q has adequate concurrency with the gold standard measurements for viral load measurement. This test can allow VL monitoring of patients on ART at the point of care in resource-limited settings.
- Performance of the SAMBA I and II HIV-1 Semi-Q Tests for viral load monitoring at the point-of-care.
- Authors: Goel N, Ritchie AV, Mtapuri-Zinyowera S, Zeh C, Stepchenkova T, Lehga J, De Ruiter A, Farleigh LE, Edemaga D, So R, Sembongi H, Wisniewski C, Nadala L, Schito M, Lee H
- Issue date: 2017 Jun
- Implementation and operational feasibility of SAMBA I HIV-1 semi-quantitative viral load testing at the point-of-care in rural settings in Malawi and Uganda.
- Authors: Gueguen M, Nicholas S, Poulet E, Schramm B, Szumilin E, Wolters L, Wapling J, Ajule E, Rakesh A, Mwenda R, Kiyaga C, Balkan S
- Issue date: 2021 Feb
- Evaluation of the Whole-Blood Alere Q NAT Point-of-Care RNA Assay for HIV-1 Viral Load Monitoring in a Primary Health Care Setting in Mozambique.
- Authors: Jani IV, Meggi B, Vubil A, Sitoe NE, Bhatt N, Tobaiwa O, Quevedo JI, Loquiha O, Lehe JD, Vojnov L, Peter TF
- Issue date: 2016 Aug
- Leukodepletion as a point-of-care method for monitoring HIV-1 viral load in whole blood.
- Authors: Titchmarsh L, Zeh C, Verpoort T, Allain JP, Lee H
- Issue date: 2015 Apr
- Prospective evaluation of diagnostic accuracy of dried blood spots from finger prick samples for determination of HIV-1 load with the NucliSENS Easy-Q HIV-1 version 2.0 assay in Malawi.
- Authors: Fajardo E, Metcalf CA, Chaillet P, Aleixo L, Pannus P, Panunzi I, Triviño L, Ellman T, Likaka A, Mwenda R
- Issue date: 2014 May