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    Jan 19, 2021
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    SAMBA HIV semi-quantitative test, a new point-of-care viral load monitoring assay for resource-limited settings

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    Ritchie et al-2014-SAMBA HIV ...
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    Authors
    Ritchie, Allyson V
    Ushiro-Lumb, Ines
    Edemaga, Daniel
    Joshi, Hrishikesh A
    De Ruiter, Annemiek
    Szumilin, Elisabeth
    Jendrulek, Isabelle
    McGuire, Megan
    Goel, Neha
    Sharma, Pia I
    Allain, Jean-Pierre
    Lee, Helen H
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    Issue Date
    2014-07-16
    
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    Journal
    Journal of clinical microbiology
    Abstract
    Routine viral load (VL) testing of HIV-infected individuals on antiretroviral therapy (ART) is used to monitor treatment efficacy. However, due to logistical challenges, implementation of VL has been difficult in resource-limited settings. The aim of this study was to evaluate the performance of the SAMBA Semi-Q Test in London, Malawi, and Uganda. The SAMBA HIV-1 Semi-Q Test can distinguish between patients with VL above or below 1000 copies/ml. The SAMBA Semi-Q was validated with diluted clinical samples and blinded plasma samples collected from HIV-1-positive individuals. SAMBA Semi-Q results were compared with results from the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Test v2.0. Testing of 96 2-10 fold dilutions of four samples containing HIV-1 subtype C as well as 488 samples from patients in the United Kingdom, Malawi, and Uganda, respectively, yielded an overall accuracy for SAMBA Semi-Q of 99% (95% CI 93.8 - 99.9%) and 96.9% (95% CI 94.9 - 98.3%) respectively compared to Roche. Analysis of VL data from patients in Malawi and Uganda showed that the SAMBA cut-off of 1000 copies/ml appropriately distinguished treated from untreated individuals. Furthermore, analysis of the viral load of 232 patients on ART in Malawi and Uganda revealed similar patterns for virological control defined as either <1000 copies/ml (SAMBA cut-off) or <5000 copies/ml (WHO 2010 criterion). This study suggests that SAMBA Semi-Q has adequate concurrency with the gold standard measurements for viral load measurement. This test can allow VL monitoring of patients on ART at the point of care in resource-limited settings.
    URI
    http://hdl.handle.net/10144/326067
    DOI
    10.1128/JCM.00593-14
    PubMed ID
    25031444
    Language
    en
    ISSN
    1098-660X
    ae974a485f413a2113503eed53cd6c53
    10.1128/JCM.00593-14
    Scopus Count
    Collections
    HIV/AIDS

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