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    Apr 10, 2021
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    Malaria PCR Detection in Cambodian Low-Transmission Settings: Dried Blood Spots Versus Venous Blood Samples

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    Canier L - 2015 - Malaria PCR ...
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    Authors
    Canier, Lydie
    Khim, Nimol
    Kim, Saorin
    Eam, Rotha
    Khean, Chanra
    Loch, Kaknika
    Ken, Malen
    Pannus, Pieter
    Bosman, Philippe
    Stassijns, Jorgen
    Nackers, Fabienne
    Alipon, SweetC
    Char, Meng Chuor
    Chea, Nguon
    Etienne, William
    De Smet, Martin
    Kindermans, Jean-Marie
    Ménard, Didier
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    Issue Date
    2015-01-05
    
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    Journal
    The American Journal of Tropical Medicine and Hygiene
    Abstract
    In the context of malaria elimination, novel strategies for detecting very low malaria parasite densities in asymptomatic individuals are needed. One of the major limitations of the malaria parasite detection methods is the volume of blood samples being analyzed. The objective of the study was to compare the diagnostic accuracy of a malaria polymerase chain reaction assay, from dried blood spots (DBS, 5 μL) and different volumes of venous blood (50 μL, 200 μL, and 1 mL). The limit of detection of the polymerase chain reaction assay, using calibrated Plasmodium falciparum blood dilutions, showed that venous blood samples (50 μL, 200 μL, 1 mL) combined with Qiagen extraction methods gave a similar threshold of 100 parasites/mL, ∼100-fold lower than 5 μL DBS/Instagene method. On a set of 521 field samples, collected in two different transmission areas in northern Cambodia, no significant difference in the proportion of parasite carriers, regardless of the methods used was found. The 5 μL DBS method missed 27% of the samples detected by the 1 mL venous blood method, but most of the missed parasites carriers were infected by Plasmodium vivax (84%). The remaining missed P. falciparum parasite carriers (N = 3) were only detected in high-transmission areas.
    Publisher
    American Society of Tropical Medicine and Hygiene
    URI
    http://hdl.handle.net/10144/344014
    DOI
    10.4269/ajtmh.14-0614
    PubMed ID
    25561570
    Additional Links
    http://www.ajtmh.org
    Language
    en
    ISSN
    1476-1645
    ae974a485f413a2113503eed53cd6c53
    10.4269/ajtmh.14-0614
    Scopus Count
    Collections
    Malaria

    entitlement

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