Malaria PCR Detection in Cambodian Low-Transmission Settings: Dried Blood Spots Versus Venous Blood Samples
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Canier L - 2015 - Malaria PCR ...
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Authors
Canier, LydieKhim, Nimol
Kim, Saorin
Eam, Rotha
Khean, Chanra
Loch, Kaknika
Ken, Malen
Pannus, Pieter
Bosman, Philippe
Stassijns, Jorgen
Nackers, Fabienne
Alipon, SweetC
Char, Meng Chuor
Chea, Nguon
Etienne, William
De Smet, Martin
Kindermans, Jean-Marie
Ménard, Didier
Issue Date
2015-01-05
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In the context of malaria elimination, novel strategies for detecting very low malaria parasite densities in asymptomatic individuals are needed. One of the major limitations of the malaria parasite detection methods is the volume of blood samples being analyzed. The objective of the study was to compare the diagnostic accuracy of a malaria polymerase chain reaction assay, from dried blood spots (DBS, 5 μL) and different volumes of venous blood (50 μL, 200 μL, and 1 mL). The limit of detection of the polymerase chain reaction assay, using calibrated Plasmodium falciparum blood dilutions, showed that venous blood samples (50 μL, 200 μL, 1 mL) combined with Qiagen extraction methods gave a similar threshold of 100 parasites/mL, ∼100-fold lower than 5 μL DBS/Instagene method. On a set of 521 field samples, collected in two different transmission areas in northern Cambodia, no significant difference in the proportion of parasite carriers, regardless of the methods used was found. The 5 μL DBS method missed 27% of the samples detected by the 1 mL venous blood method, but most of the missed parasites carriers were infected by Plasmodium vivax (84%). The remaining missed P. falciparum parasite carriers (N = 3) were only detected in high-transmission areas.PubMed ID
25561570Additional Links
http://www.ajtmh.orgLanguage
enISSN
1476-1645ae974a485f413a2113503eed53cd6c53
10.4269/ajtmh.14-0614
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