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    Jan 16, 2021
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    Field Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greece

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    Tseroni et al - 2015 - Field ...
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    Authors
    Tseroni, Maria
    Pervanidou, Danai
    Tserkezou, Persefoni
    Rachiotis, George
    Pinaka, Ourania
    Baka, Agoritsa
    Georgakopoulou, Theano
    Vakali, Annita
    Dionysopoulou, Martha
    Terzaki, Irene
    Marka, Andriani
    Detsis, Marios
    Evlampidou, Zafiroula
    Mpimpa, Anastasia
    Vassalou, Evdokia
    Tsiodras, Sotirios
    Tsakris, Athanasios
    Kremastinou, Jenny
    Hadjichristodoulou, Christos
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    Issue Date
    2015-03-24
    
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    Journal
    PloS One
    Abstract
    Greece, a malaria-free country since 1974, has experienced re-emergence of Plasmodium vivax autochthonous malaria cases in some agriculture areas over the last three years. In early 2012, an integrated control programme (MALWEST Project) was launched in order to prevent re-establishment of the disease. In the context of this project, the rapid diagnostic tests (RDT) of SD Bioline Malaria Ag Pf/Pan that detects hrp-2 and pan-LDH antigens were used. The aim of this study was to assess the field application of the RDT for the P. vivax diagnosis in comparison to light microscopy and polymerase chain reaction (PCR). A total of 955 samples were tested with all three diagnostic tools. Agreement of RDT against microscopy and PCR for the diagnosis of P. vivax was satisfactory (K value: 0.849 and 0.976, respectively). The sensitivity, specificity and positive predictive value of RDT against PCR was 95.6% (95% C.I.: 84.8-99.3), 100% (95% C.I.: 99.6-100.0) and 100% (95% CI: 91.7-100.0) respectively, while the sensitivity, specificity and positive predictive value of RDT against microscopic examination was 97.4% (95% C.I.: 86.1-99.6), 99.4% (95% C.I.: 98.6-99.8) and 86.1% (95% CI: 72.1-94.7), respectively. Our results indicate that RDT performed satisfactory in a non-endemic country and therefore is recommended for malaria diagnosis, especially in areas where health professionals lack experience on light microscopy.
    Publisher
    Public Library of Science
    URI
    http://hdl.handle.net/10144/550439
    DOI
    10.1371/journal.pone.0120367
    PubMed ID
    25803815
    Language
    en
    ISSN
    1932-6203
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0120367
    Scopus Count
    Collections
    Malaria

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