Field Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greece
Authors
Tseroni, MariaPervanidou, Danai
Tserkezou, Persefoni
Rachiotis, George
Pinaka, Ourania
Baka, Agoritsa
Georgakopoulou, Theano
Vakali, Annita
Dionysopoulou, Martha
Terzaki, Irene
Marka, Andriani
Detsis, Marios
Evlampidou, Zafiroula
Mpimpa, Anastasia
Vassalou, Evdokia
Tsiodras, Sotirios
Tsakris, Athanasios
Kremastinou, Jenny
Hadjichristodoulou, Christos
Issue Date
2015-03-24
Metadata
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PloS OneAbstract
Greece, a malaria-free country since 1974, has experienced re-emergence of Plasmodium vivax autochthonous malaria cases in some agriculture areas over the last three years. In early 2012, an integrated control programme (MALWEST Project) was launched in order to prevent re-establishment of the disease. In the context of this project, the rapid diagnostic tests (RDT) of SD Bioline Malaria Ag Pf/Pan that detects hrp-2 and pan-LDH antigens were used. The aim of this study was to assess the field application of the RDT for the P. vivax diagnosis in comparison to light microscopy and polymerase chain reaction (PCR). A total of 955 samples were tested with all three diagnostic tools. Agreement of RDT against microscopy and PCR for the diagnosis of P. vivax was satisfactory (K value: 0.849 and 0.976, respectively). The sensitivity, specificity and positive predictive value of RDT against PCR was 95.6% (95% C.I.: 84.8-99.3), 100% (95% C.I.: 99.6-100.0) and 100% (95% CI: 91.7-100.0) respectively, while the sensitivity, specificity and positive predictive value of RDT against microscopic examination was 97.4% (95% C.I.: 86.1-99.6), 99.4% (95% C.I.: 98.6-99.8) and 86.1% (95% CI: 72.1-94.7), respectively. Our results indicate that RDT performed satisfactory in a non-endemic country and therefore is recommended for malaria diagnosis, especially in areas where health professionals lack experience on light microscopy.Publisher
Public Library of SciencePubMed ID
25803815Language
enISSN
1932-6203ae974a485f413a2113503eed53cd6c53
10.1371/journal.pone.0120367
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