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dc.contributor.authorTseroni, Maria
dc.contributor.authorPervanidou, Danai
dc.contributor.authorTserkezou, Persefoni
dc.contributor.authorRachiotis, George
dc.contributor.authorPinaka, Ourania
dc.contributor.authorBaka, Agoritsa
dc.contributor.authorGeorgakopoulou, Theano
dc.contributor.authorVakali, Annita
dc.contributor.authorDionysopoulou, Martha
dc.contributor.authorTerzaki, Irene
dc.contributor.authorMarka, Andriani
dc.contributor.authorDetsis, Marios
dc.contributor.authorEvlampidou, Zafiroula
dc.contributor.authorMpimpa, Anastasia
dc.contributor.authorVassalou, Evdokia
dc.contributor.authorTsiodras, Sotirios
dc.contributor.authorTsakris, Athanasios
dc.contributor.authorKremastinou, Jenny
dc.contributor.authorHadjichristodoulou, Christos
dc.date.accessioned2015-04-21T18:27:40Z
dc.date.available2015-04-21T18:27:40Z
dc.date.issued2015-03-24
dc.identifier.citationField Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greece. 2015, 10 (3):e0120367 PLoS ONEen_GB
dc.identifier.issn1932-6203
dc.identifier.pmid25803815
dc.identifier.doi10.1371/journal.pone.0120367
dc.identifier.urihttp://hdl.handle.net/10144/550439
dc.description.abstractGreece, a malaria-free country since 1974, has experienced re-emergence of Plasmodium vivax autochthonous malaria cases in some agriculture areas over the last three years. In early 2012, an integrated control programme (MALWEST Project) was launched in order to prevent re-establishment of the disease. In the context of this project, the rapid diagnostic tests (RDT) of SD Bioline Malaria Ag Pf/Pan that detects hrp-2 and pan-LDH antigens were used. The aim of this study was to assess the field application of the RDT for the P. vivax diagnosis in comparison to light microscopy and polymerase chain reaction (PCR). A total of 955 samples were tested with all three diagnostic tools. Agreement of RDT against microscopy and PCR for the diagnosis of P. vivax was satisfactory (K value: 0.849 and 0.976, respectively). The sensitivity, specificity and positive predictive value of RDT against PCR was 95.6% (95% C.I.: 84.8-99.3), 100% (95% C.I.: 99.6-100.0) and 100% (95% CI: 91.7-100.0) respectively, while the sensitivity, specificity and positive predictive value of RDT against microscopic examination was 97.4% (95% C.I.: 86.1-99.6), 99.4% (95% C.I.: 98.6-99.8) and 86.1% (95% CI: 72.1-94.7), respectively. Our results indicate that RDT performed satisfactory in a non-endemic country and therefore is recommended for malaria diagnosis, especially in areas where health professionals lack experience on light microscopy.
dc.language.isoenen
dc.publisherPublic Library of Scienceen_GB
dc.rightsPublished by Public Library of Science, [url]http://www.plosone.org/[/url] Archived on this site by Open Access permissionen_GB
dc.titleField Application of SD Bioline Malaria Ag Pf/Pan Rapid Diagnostic Test for Malaria in Greeceen
dc.identifier.journalPloS Oneen_GB
refterms.dateFOA2019-03-04T12:19:01Z
html.description.abstractGreece, a malaria-free country since 1974, has experienced re-emergence of Plasmodium vivax autochthonous malaria cases in some agriculture areas over the last three years. In early 2012, an integrated control programme (MALWEST Project) was launched in order to prevent re-establishment of the disease. In the context of this project, the rapid diagnostic tests (RDT) of SD Bioline Malaria Ag Pf/Pan that detects hrp-2 and pan-LDH antigens were used. The aim of this study was to assess the field application of the RDT for the P. vivax diagnosis in comparison to light microscopy and polymerase chain reaction (PCR). A total of 955 samples were tested with all three diagnostic tools. Agreement of RDT against microscopy and PCR for the diagnosis of P. vivax was satisfactory (K value: 0.849 and 0.976, respectively). The sensitivity, specificity and positive predictive value of RDT against PCR was 95.6% (95% C.I.: 84.8-99.3), 100% (95% C.I.: 99.6-100.0) and 100% (95% CI: 91.7-100.0) respectively, while the sensitivity, specificity and positive predictive value of RDT against microscopic examination was 97.4% (95% C.I.: 86.1-99.6), 99.4% (95% C.I.: 98.6-99.8) and 86.1% (95% CI: 72.1-94.7), respectively. Our results indicate that RDT performed satisfactory in a non-endemic country and therefore is recommended for malaria diagnosis, especially in areas where health professionals lack experience on light microscopy.


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