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dc.contributor.authorPark, Daniel J
dc.contributor.authorDudas, Gytis
dc.contributor.authorWohl, Shirlee
dc.contributor.authorGoba, Augustine
dc.contributor.authorWhitmer, Shannon L M
dc.contributor.authorAndersen, Kristian G
dc.contributor.authorSealfon, Rachel S
dc.contributor.authorLadner, Jason T
dc.contributor.authorKugelman, Jeffrey R
dc.contributor.authorMatranga, Christian B
dc.contributor.authorWinnicki, Sarah M
dc.contributor.authorQu, James
dc.contributor.authorGire, Stephen K
dc.contributor.authorGladden-Young, Adrianne
dc.contributor.authorJalloh, Simbirie
dc.contributor.authorNosamiefan, Dolo
dc.contributor.authorYozwiak, Nathan L
dc.contributor.authorMoses, Lina M
dc.contributor.authorJiang, Pan-Pan
dc.contributor.authorLin, Aaron E
dc.contributor.authorSchaffner, Stephen F
dc.contributor.authorBird, Brian
dc.contributor.authorTowner, Jonathan
dc.contributor.authorMamoh, Mambu
dc.contributor.authorGbakie, Michael
dc.contributor.authorKanneh, Lansana
dc.contributor.authorKargbo, David
dc.contributor.authorMassally, James L B
dc.contributor.authorKamara, Fatima K
dc.contributor.authorKonuwa, Edwin
dc.contributor.authorSellu, Josephine
dc.contributor.authorJalloh, Abdul A
dc.contributor.authorMustapha, Ibrahim
dc.contributor.authorFoday, Momoh
dc.contributor.authorYillah, Mohamed
dc.contributor.authorErickson, Bobbie R
dc.contributor.authorSealy, Tara
dc.contributor.authorBlau, Dianna
dc.contributor.authorPaddock, Christopher
dc.contributor.authorBrault, Aaron
dc.contributor.authorAmman, Brian
dc.contributor.authorBasile, Jane
dc.contributor.authorBearden, Scott
dc.contributor.authorBelser, Jessica
dc.contributor.authorBergeron, Eric
dc.contributor.authorCampbell, Shelley
dc.contributor.authorChakrabarti, Ayan
dc.contributor.authorDodd, Kimberly
dc.contributor.authorFlint, Mike
dc.contributor.authorGibbons, Aridth
dc.contributor.authorGoodman, Christin
dc.contributor.authorKlena, John
dc.contributor.authorMcMullan, Laura
dc.contributor.authorMorgan, Laura
dc.contributor.authorRussell, Brandy
dc.contributor.authorSalzer, Johanna
dc.contributor.authorSanchez, Angela
dc.contributor.authorWang, David
dc.contributor.authorJungreis, Irwin
dc.contributor.authorTomkins-Tinch, Christopher
dc.contributor.authorKislyuk, Andrey
dc.contributor.authorLin, Michael F
dc.contributor.authorChapman, Sinead
dc.contributor.authorMacInnis, Bronwyn
dc.contributor.authorMatthews, Ashley
dc.contributor.authorBochicchio, James
dc.contributor.authorHensley, Lisa E
dc.contributor.authorKuhn, Jens H
dc.contributor.authorNusbaum, Chad
dc.contributor.authorSchieffelin, John S
dc.contributor.authorBirren, Bruce W
dc.contributor.authorForget, Marc
dc.contributor.authorNichol, Stuart T
dc.contributor.authorPalacios, Gustavo F
dc.contributor.authorNdiaye, Daouda
dc.contributor.authorHappi, Christian
dc.contributor.authorGevao, Sahr M
dc.contributor.authorVandi, Mohamed A
dc.contributor.authorKargbo, Brima
dc.contributor.authorHolmes, Edward C
dc.contributor.authorBedford, Trevor
dc.contributor.authorGnirke, Andreas
dc.contributor.authorStröher, Ute
dc.contributor.authorRambaut, Andrew
dc.contributor.authorGarry, Robert F
dc.contributor.authorSabeti, Pardis C
dc.date.accessioned2015-08-18T21:27:33Z
dc.date.available2015-08-18T21:27:33Z
dc.date.issued2015-06-18
dc.identifier.citationEbola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone. 2015, 161 (7):1516-26 Cellen_GB
dc.identifier.issn1097-4172
dc.identifier.pmid26091036
dc.identifier.doi10.1016/j.cell.2015.06.007
dc.identifier.urihttp://hdl.handle.net/10144/575207
dc.description.abstractThe 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.
dc.language.isoenen
dc.rightsArchived with thanks to Cell's open accessen_GB
dc.titleEbola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leoneen
dc.identifier.journalCellen_GB
refterms.dateFOA2019-03-04T12:32:30Z
html.description.abstractThe 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.


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