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    Apr 17, 2021
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    Cholera Rapid Test with Enrichment Step Has Diagnostic Performance Equivalent to Culture

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    Ontweka LN et al - 2016 - Cholera ...
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    Authors
    Ontweka, LN
    Deng, LO
    Rauzier, J
    Debes, AK
    Tadesse, F
    Parker, LA
    Wamala, JF
    Bior, BK
    Lasuba, M
    But, AB
    Grandesso, F
    Jamet, C
    Cohuet, S
    Ciglenecki, I
    Serafini, M
    Sack, DA
    Quilici, ML
    Azman, AS
    Luquero, FJ
    Page, AL
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    Issue Date
    2016-12-19
    Submitted date
    2017-01-11
    
    Metadata
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    Journal
    PloS One
    Abstract
    Cholera rapid diagnostic tests (RDT) could play a central role in outbreak detection and surveillance in low-resource settings, but their modest performance has hindered their broad adoption. The addition of an enrichment step may improve test specificity. We describe the results of a prospective diagnostic evaluation of the Crystal VC RDT (Span Diagnostics, India) with enrichment step and of culture, each compared to polymerase chain reaction (PCR), during a cholera outbreak in South Sudan. RDTs were performed on alkaline peptone water inoculated with stool and incubated for 4-6 hours at ambient temperature. Cholera culture was performed from wet filter paper inoculated with stool. Molecular detection of Vibrio cholerae O1 by PCR was done from dry Whatman 903 filter papers inoculated with stool, and from wet filter paper supernatant. In August and September 2015, 101 consecutive suspected cholera cases were enrolled, of which 36 were confirmed by PCR. The enriched RDT had 86.1% (95% CI: 70.5-95.3) sensitivity and 100% (95% CI: 94.4-100) specificity compared to PCR as the reference standard. The sensitivity of culture versus PCR was 83.3% (95% CI: 67.2-93.6) for culture performed on site and 72.2% (95% CI: 54.8-85.8) at the international reference laboratory, where samples were tested after an average delay of two months after sample collection, and specificity was 98.5% (95% CI: 91.7-100) and 100% (95% CI: 94.5-100), respectively. The RDT with enrichment showed performance comparable to that of culture and could be a sustainable alternative to culture confirmation where laboratory capacity is limited.
    Publisher
    Public Library of Science
    URI
    http://hdl.handle.net/10144/618744
    DOI
    10.1371/journal.pone.0168257
    PubMed ID
    27992488
    Language
    en
    ISSN
    1932-6203
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0168257
    Scopus Count
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