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dc.contributor.authorArdizzoni, E
dc.contributor.authorOrikiriza, P
dc.contributor.authorSsuuna, C
dc.contributor.authorNyehangane, D
dc.contributor.authorGumsboga, M
dc.contributor.authorTaremwa, IM
dc.contributor.authorTuryashemererwa, E
dc.contributor.authorMwanga-Amumpaire, J
dc.contributor.authorLangendorf, C
dc.contributor.authorBonnet, M
dc.date.accessioned2019-11-12T12:55:46Z
dc.date.available2019-11-12T12:55:46Z
dc.date.issued2019-10-16
dc.date.submitted2019-11-08
dc.identifier.urihttp://hdl.handle.net/10144/619501
dc.description.abstractBackground: Xpert MTB/RIF (Xpert) and culture are the most reliable methods for tuberculosis diagnosis but are still poorly accessible in many low resource countries. We aimed to assess the effect of OMNIgene® SPUTUM (OM-S) and ethanol in preserving sputum for Xpert and OM-S for mycobacteria growth indicator tube (MGIT) testing over a period of 15 and 8 days respectively. Methods: Sputum were collected from newly diagnosed smear-positive patients. For Xpert, pooled samples were split into 5 aliquots: 3 for Xpert on day 0, 7 and 15 days without additive and 2 with either OM-S or ethanol at day 15. For MGIT, 2 aliquots were tested without preservative and 2 with OM-S at 0 and 8 days. Results: A total of 48 and 47 samples were included in the analysis for Xpert and culture. With Xpert, using Day 0 as reference, untreated samples stored for 7 and 15 days showed concordance of 45/46 (97.8%) and 46/48 (95.8%). For samples preserved with OM-S or ethanol for 15 days compared with untreated samples processed at day 0 or after 15 days, OM-S concordance was 46/48(95.8%) and 47/48(97.9%), while ethanol was 44/48 (91.7%) and 45/48 (93.8%). With MGIT, concordance between untreated and OM-S treated samples was 21/41(51.2%) at Day 0 and 21/44(47.7%) at day8. Conclusions: Xpert equally detected TB in OM-S treated and untreated samples up to 15 days but showed slightly lower detection in ethanol treated samples. Among OM-S treated samples, MGIT positivity was significantly lower compared to untreated samples at both time-points.en_US
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsWith thanks to the American Society for Microbiology.en_US
dc.titleEvaluation of OMNIgene® SPUTUM and ethanol reagent for preservation of sputum prior to Xpert and culture testing in Uganda.en_US
dc.identifier.journalJournal of Clinical Microbiologyen_US
refterms.dateFOA2019-11-12T12:55:46Z


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